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Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Immunohistochemistry
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
E. coli-derived recombinant mouse Stella/Dppa3
Met1-Asn150
Accession # Q8QZY3
Met1-Asn150
Accession # Q8QZY3
Specificity
Detects mouse Stella/Dppa3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross‑reactivity with recombinant human Stella is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse Stella/Dppa3 Antibody
Stella/Dppa3 in mouse ovary tissue.
Stella/Dppa3 was detected in perfusion fixed frozen sections of mouse ovary tissue using Goat Anti-Mouse Stella/Dppa3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2566) at 1 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (brown; Catalog # VC004) and counterstained with hematoxylin (blue). Specific staining was localized to oocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of Stella/Dppa3 by Western Blot
Reduced expression of Dppa3 in Zfp296-KO ESCs.a Immunofuorescence analysis of DPPA3 expression in WT, Zfp296-KO #98, and Rescue #22 ESCs. Nuclei were counterstained with DAPI. Scale bars, 50 μm (upper panels), 25 μm (middle and lower panels). b Western blot analysis of DPPA3 expression in WT, Rescue #22, and KO #98 ESCs (upper panel). Signal intensity of each DPPA3 band was measured relative to that of the Lamin B1 band. The lower panel shows the DPPA3 levels expressed relative to those in WT ESCs and as means ± SD (n = 3). *P < 0.05 by Tukey’s test. c Analysis of the DNA methylation levels of the Dppa3 promoter. Genomic DNA was isolated from WT, KO #98 and #156, and Rescue #22 and #10 ESCs. The CpG islands in the upstream region of the Dppa3 gene were analyzed for cytosine methylation. Filled and open circles represent methylated and unmethylated CpGs, respectively. The percentage of methylated CpG sites is shown under each panel. d Analysis of 5mC and 5hmC levels by dot blot assay. Control 5mC and 5hmC DNA and genomic DNA from WT, KO #98 and #156, and Rescue #22 and #10 ESCs were denatured. Two-fold serially diluted DNAs were spotted onto membranes. Antibody against 5mC (left panel) or 5hmC (right panel) was utilized for detection. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37488353), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Stella/Dppa3 by Immunocytochemistry/ Immunofluorescence
Reduced expression of Dppa3 in Zfp296-KO ESCs.a Immunofuorescence analysis of DPPA3 expression in WT, Zfp296-KO #98, and Rescue #22 ESCs. Nuclei were counterstained with DAPI. Scale bars, 50 μm (upper panels), 25 μm (middle and lower panels). b Western blot analysis of DPPA3 expression in WT, Rescue #22, and KO #98 ESCs (upper panel). Signal intensity of each DPPA3 band was measured relative to that of the Lamin B1 band. The lower panel shows the DPPA3 levels expressed relative to those in WT ESCs and as means ± SD (n = 3). *P < 0.05 by Tukey’s test. c Analysis of the DNA methylation levels of the Dppa3 promoter. Genomic DNA was isolated from WT, KO #98 and #156, and Rescue #22 and #10 ESCs. The CpG islands in the upstream region of the Dppa3 gene were analyzed for cytosine methylation. Filled and open circles represent methylated and unmethylated CpGs, respectively. The percentage of methylated CpG sites is shown under each panel. d Analysis of 5mC and 5hmC levels by dot blot assay. Control 5mC and 5hmC DNA and genomic DNA from WT, KO #98 and #156, and Rescue #22 and #10 ESCs were denatured. Two-fold serially diluted DNAs were spotted onto membranes. Antibody against 5mC (left panel) or 5hmC (right panel) was utilized for detection. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37488353), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Mouse Stella/Dppa3 Antibody
Application
Recommended Usage
Immunohistochemistry
1-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse ovary
Sample: Perfusion fixed frozen sections of mouse ovary
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse Stella/Dppa3
Sample: Recombinant Mouse Stella/Dppa3
Reviewed Applications
Read 2 reviews rated 4.5 using AF2566 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Stella/Dppa3
Long Name
Developmental Pluripotency-associated Protein 3
Alternate Names
Dppa3
Gene Symbol
DPPA3
UniProt
Additional Stella/Dppa3 Products
Product Documents for Mouse Stella/Dppa3 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse Stella/Dppa3 Antibody
For research use only
Related Research Areas
Citations for Mouse Stella/Dppa3 Antibody
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Customer Reviews for Mouse Stella/Dppa3 Antibody (2)
4.5 out of 5
2 Customer Ratings
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Application: ImmunohistochemistrySample Tested: Ovary tissueSpecies: MouseVerified Customer | Posted 11/15/2024Working concentration 1:200, IHC-P
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Application: Immunocytochemistry/ImmunofluorescenceSample Tested: Ovary tissueSpecies: MouseVerified Customer | Posted 11/08/2024The antibody was tested on a paraffin-embedded section of a mouse P14 ovary, with successful staining observed at a dilution of 1:200. Yellow (Nr2F2), Magenta (Stella)
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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