Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry, Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 129502
View all TGF-beta RII Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse TGF-beta RII
Ile24-Asp184
Accession # Q62312

Specificity

Detects mouse TGF-beta RII in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with with recombinant rat MIS RII, recombinant mouse TGF-beta RI, recombinant human (rh) TGF-beta RII, rhTGF-beta RIIb, or rhTGF-beta RIII.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse TGF-beta RII Antibody (129502)

Detection of Mouse TGF-beta RII by Western Blot

Detection of Mouse TGF-beta RII by Western Blot

Differential Role of Cathepsins in UUO-Induced Kidney Fibrosis(A) Immunofluorescent staining of epithelial cell marker ZO-1 (green) with nuclei counterstained with DAPI (blue) in kidneys from sham WT or 14 days post-UUO WT, Ctsb−/−, Ctsl−/−, Ctss−/−, and Ctsk−/− mice and quantification of positive areas.(B) Immunoblot analysis of TGFBR1, TGFBR2, pSmad-2, and pSmad-3 and quantification relative to GAPDH in kidney extracts from sham WT or 14 days post-UUO WT, Ctsb−/−, and Ctsl−/− mice.(C) Immunoblot analysis of TGFBR1, TGFBR2, pSmad-2, and pSmad-3 and quantification relative to GAPDH in kidney extracts from sham WT or 14 days post-UUO WT, Ctss−/−, and Ctsk−/− mice.(D) Immunostaining of pSmad-2 and pSmad-3 in kidneys from the same sets of mice and quantification of positive areas.Scale in A: 100 μm. Scale in D: 50 μm. n = 8–10 per group. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31446224), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse TGF-beta RII Antibody (129502)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse TGF-beta  RII Fc Chimera (Catalog # 1600-R2)

Reviewed Applications

Read 2 reviews rated 3 using MAB532 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TGF-beta RII

TGF-beta RII is a membrane-bound serine/threonine kinase. It associates with TGF-beta RI to generate a heterodimeric signaling complex that transduces TGF-beta signals. This receptor binds TGF-beta 1 and TGF-beta 3 with high affinity and TGF-beta 2 with much lower affinity and is expressed by most cell types.

Long Name

Transforming Growth Factor beta Receptor II

Alternate Names

TGF-bRII, TGFbetaRII, TGFBR2

Entrez Gene IDs

7048 (Human); 21813 (Mouse)

Gene Symbol

TGFBR2

UniProt

Q62312

Additional TGF-beta RII Products

Product Documents for Mouse TGF-beta RII Antibody (129502)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse TGF-beta RII Antibody (129502)

For research use only

Citations for Mouse TGF-beta RII Antibody (129502)

Customer Reviews for Mouse TGF-beta RII Antibody (129502) (2)

3 out of 5
2 Customer Ratings
5 Stars
50%
4 Stars
0%
3 Stars
0%
2 Stars
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1 Stars
50%

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Customer Images

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  • Mouse TGF-beta  RII Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Recombinant protein
    Species: Mouse
    Verified Customer | Posted 04/07/2020
    Mouse TGF-beta RII Antibody (129502) MAB532
  • Mouse TGF-beta  RII Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: spinal cord sections
    Species: Mouse
    Verified Customer | Posted 12/09/2016
    This customer tested this product in an application which had not been validated and qualified for our Innovator's Reward Program to earn a voucher for 100% the purchase price. I have gotten this antibody to work at a 1:250 dilution on mouse frozen spinal cord tissue (see attached.jpg, DAPI in blue, TGFBR2 in green). Regrettably I cannot use this antibody because it stains in a completely different pattern from NB100-91994, the accuracy of which I’ve verified through transfection experiments.
    Mouse TGF-beta RII Antibody (129502) MAB532

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