Mouse TIM-4 Antibody

R&D Systems | Catalog # AF2826

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all TIM-4 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse TIM-4
Ala22-Thr279
Accession # Q6U7R4

Specificity

Detects mouse TIM-4 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross‑reactivity with recombinant human (rh) TIM-1, recombinant mouse (rm) TIM-2, rmTIM-3, rhTIM-4, rmTIM-5, and rmTIM-6 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse TIM-4 Antibody

Detection of TIM-4 in Mouse Embryonic Liver Cryosections.

Detection of TIM-4 in Mouse Embryonic Liver Cryosections.

Stained embryonic liver cryosections for Tim4 (red) and Hoechst (blue). Image from a verified customer review.
Detection of TIM-4 by Western Blot

Detection of TIM-4 by Western Blot

Tim-4 inhibited NO production by NF-kappa B signaling pathway.RAW-pcDNA3-Tim-4 and RAW-pcDNA3 cells were stimulated with LPS for the times indicated and western blot was used to detect phosphorylation of NF-kappa B p65 (A). Production of NO in LPS-activated RAW264.7 was detected before or after treatment with NF-kappa B inhibitory ligand (B). Peritoneal macrophages were pre-treated with goat anti-mouse Tim-4 antibody or control IgG at 5 μg/ml for 1 h, and then stimulated with 100 ng/ml LPS for 30 min. Cells were harvested for western blot (C). Production of NO in LPS-stimulated peritoneal macrophages with or without anti-Tim-4 pretreatment was detected before or after application of NF-kappa B inhibitory ligand (D). These experiments were repeated at least 3 times. Error bars represent the standard deviation from 4 independent cultures from 4 mice. A p value (student’s t-test) relative to the control cells of less than 0.01 is indicated by **. ns represents a p value greater than 0.05. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25905790), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse TIM-4 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse TIM‑4 (Catalog # 2826-TI)

Reviewed Applications

Read 1 review rated 5 using AF2826 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TIM-4

TIM-4 (T cell; immunoglobulin; mucin-4), also known as SMUCKLER, is a 60 kDa member of the TIM family of immune regulating proteins. TIMs are type I transmembrane proteins with one Ig-like V domain and one Ser/Thr-rich mucin domain (1 - 3). The mouse TIM-4 cDNA encodes a 343 amino acid (aa) precursor that includes a 22 aa signal sequence, a 257 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 43 aa cytoplasmic tail (4). Structurally, TIM-4 is distinguished from other TIMs by the presence of an RGD motif in its Ig domain and the lack of a site for tyrosine phosphorylation in its cytoplasmic tail. The mucin domain in TIM-4 is larger than in TIM-1, -2, or -3. Within the ECD, mouse TIM-4 shares 27 - 33% aa sequence identity with mouse TIM-1, -2, and -3. The ECD of mouse TIM-4 shares 45% aa sequence identity with that of human and rat TIM-4. TIM-4 is expressed by macrophages and mature dendritic cells but not by lymphocytes (4, 5). TIM-4 binds specifically to TIM-1 which is also the cellular receptor for hepatitis A virus and has been implicated in the development of asthma (5 ‑ 7). Among hematopoietic cells, TIM-1 is expressed on activated B and T cells, preferentially in the Th2 subset of CD4+ T cells (5, 8). The interaction of TIM-4 with TIM-1 induces co-stimulatory and hyperproliferative signals in T cells (5).

References

  1. Kuchroo, V.K. et al. (2003) Nat. Rev. Immunol. 3:454. 
  2. Mariat, C. et al. (2005) Phil. Trans. R. Soc. B 360:1681.  
  3. Meyers, J.H. et al. (2005) Trends Mol. Med. 11:362. 
  4. Shakhov, A.N. et al. (2004) Eur. J. Immunol. 34:494. 
  5. Meyers, J.H. et al. (2005) Nat. Immunol. 6:455.
  6. Feigelstock, D. et al. (1998) J. Virol. 72:6621.
  7. McIntire, J.J. et al. (2001) Nat. Immunol. 2:1109.
  8. Khademi, M. et al. (2004) J. Immunol. 172:7169.

Long Name

T Cell Immunoglobulin Mucin-4

Alternate Names

Smuckler, TIM4, TIMD4

Entrez Gene IDs

91937 (Human); 276891 (Mouse); 102140672 (Cynomolgus Monkey)

Gene Symbol

TIMD4

UniProt

Q6U7R4

Additional TIM-4 Products

Product Documents for Mouse TIM-4 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse TIM-4 Antibody

For research use only

Citations for Mouse TIM-4 Antibody

Customer Reviews for Mouse TIM-4 Antibody (1)

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Customer Images

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  • Mouse TIM-4 Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: Embryonic liver
    Species: Mouse
    Verified Customer | Posted 09/25/2025
    Stained embryonic liver cryosections for Tim4 (red) and Hoechst (blue). Tim4 works great every time.
    Mouse TIM-4 Antibody AF2826
    Bio-Techne Response
    This review reflects a new species or application tested on a primary antibody.

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