Mouse TNF RI/TNFRSF1A Antibody (55R170)

R&D Systems | Catalog # MAB430

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Neutralization, Immunoprecipitation

Cited:

Immunohistochemistry, Western Blot, Neutralization, Immunocytochemistry, Immunoprecipitation, Cell Culture

Label

Unconjugated

Antibody Source

Monoclonal Hamster IgG Clone # 55R170
View all TNF RI/TNFRSF1A Primary Antibodies »

Product Specifications

Immunogen

Recombinant mouse TNF RI/TNFRSF1A
Extracellular domain

Specificity

Detects mouse TNF RI/TNFRSF1A in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human(rh) 4‑1BB, rhCD27, rhCD30, recombinant mouse (rm) CD27, rmCD30, rhCD40, rmCD40, rhDR3, rhDR6, rmEDAR, rhFas, rmFas, rhEDAR, rhGITR, rmGITR, rhHVEM, rhLT beta R, rmLT beta R, rhNGF R, rhOPG, rmOPG, rhRANK, rmRANK, rhTAJ, rhTNF RI, rhTNF RII or rmTNF RII is observed.

Clonality

Monoclonal

Host

Hamster

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse TNF RI/TNFRSF1A Antibody (55R170)

Cytotoxicity Induced by TNF‑ alpha and Neutralization by Mouse TNF RI/TNFRSF1A Antibody.

Cytotoxicity Induced by TNF‑ alpha and Neutralization by Mouse TNF RI/TNFRSF1A Antibody.

Recombinant Mouse TNF-a (Catalog # 410-MT) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Mouse TNF-a (0.1 ng/mL) is neutralized (green line) by increasing concentrations of Hamster Anti-Mouse TNF RI/TNFRSF1A Monoclonal Antibody (Catalog # MAB430). The ND50 is typically 5-20 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).

Applications for Mouse TNF RI/TNFRSF1A Antibody (55R170)

Application
Recommended Usage

Immunoprecipitation

Sheehan, K. et al. (1995) J. Exp. Med. 181:607.

Western Blot

1 µg/mL
Sample: Recombinant Mouse TNF RI/TNFRSF1A (Catalog # 425-R1)
under non-reducing conditions only

Neutralization

Measured by its ability to neutralize TNF‑ alpha -induced cytotoxicity in the L‑929 mouse fibroblast cell line. Matthews, N. and M. L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M. J. et al. (eds): IRL Press. 221. The Neutralization Dose (ND50) is typically 5-20 µg/mL in the presence of 0.1 ng/mL Recombinant Mouse TNF‑ alpha and 1 µg/mL actinomycin D.

Reviewed Applications

Read 2 reviews rated 4.5 using MAB430 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TNF RI/TNFRSF1A

TNF receptor 1 (TNF RI; also called TNF R-p55/p60 and TNFRSF1A) is a type I transmembrane protein member of the TNF receptor superfamily, designated TNFRSF1A (1, 2). Both TNF RI and TNF RII (TNFRSF1B) are widely expressed and contain four TNF-alpha trimer-binding cysteine-rich domains (CRD) in their extracellular domains (ECD). However, TNF RI is thought to mediate most of the cellular effects of TNF-alpha (3). It is essential for proper development of lymph node germinal centers and Peyer’s patches, and for combating intracellular pathogens such as Listeria (1‑3). TNF RI is also a receptor for TNF-beta /TNFSF1B (lymphotoxin-alpha ) (4). TNF RI is present on the cell surface as a trimer of 55 kDa subunits (4, 5). TNF-alpha induces sequestering of TNF RI in lipid rafts, where it activates NF kappa B and is cleaved by ADAM-17/TACE (9, 10). Release of the 28 - 34 kDa TNF RI ECD also occurs constitutively and in response to products of pathogens such as LPS, CpG DNA or S. aureus protein A (1, 6‑8). Full-length TNF RI may also be released in exosome-like vesicles (11). Release helps to resolve inflammatory reactions, since it down-regulates cell surface TNF RI and provides soluble TNF RI to bind TNF-alpha (6, 12, 13). Exclusion from lipid rafts causes endocytosis of TNF RI complexes and induces apoptosis (1). Mouse TNF RI is a 454 amino acid (aa) protein that contains a 21 aa signal sequence, a 191 aa ECD with a PLAD domain (5) that mediates constitutive trimer formation, followed by the four CRD, a 23 aa transmembrane domain, and a 219 aa cytoplasmic sequence that contains a neutral sphingomyelinase activation domain and a death domain (15). The ECD of mouse TNF RI shows 67%, 70%, 64%, 70% and 88% aa identity with canine, feline, porcine, human, and rat TNF RI, respectively; it shows 23% aa identity with the ECD of TNF RII.

References

  1. Pfeffer, K. (2003) Cytokine Growth Factor Rev. 14:185.
  2. Hehlgans, T. and K. Pfeffer (2005) Immunology 115:1.
  3. Peschon, J.J. et al. (1998) J. Immunol. 160:943.
  4. Banner, D.W et al. (1993) Cell 73: 431.
  5. Chan, F.K. et al. (2000) Science 288:2351.
  6. Xanthoulea, S. et al. (2004) J. Exp. Med. 200:367.
  7. Jin, L. et al. (2000) J. Immunol. 165:5153.
  8. Gomez, M.I. et al. (2006) J. Biol. Chem. 281:20190.
  9. Legler, D.F. et al. (2003) Immunity 18:655.
  10. Tellier, E. et al. (2006) Exp. Cell Res. 312:3969.
  11. Islam, A. et al. (2006) J. Biol. Chem. 281:6860.
  12. Garton, K.J. et al. (2006) J. Leukoc. Biol. 79:1105.
  13. McDermott, M.F. et al. (1999) Cell 97:133.
  14. Schneider-Brachert, W. et al. (2004) Immunity 21:415.
  15. Lewis, M. et al. (1991) Proc. Natl. Acad. Sci. USA 88:2830.

Long Name

Tumor Necrosis Factor Receptor I

Alternate Names

CD120a, TNFRI, TNFRSF1A

Entrez Gene IDs

7132 (Human); 21937 (Mouse); 25625 (Rat); 102135285 (Cynomolgus Monkey)

Gene Symbol

TNFRSF1A

Additional TNF RI/TNFRSF1A Products

Product Documents for Mouse TNF RI/TNFRSF1A Antibody (55R170)

Certificate of Analysis

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Product Specific Notices for Mouse TNF RI/TNFRSF1A Antibody (55R170)

For research use only

Citations for Mouse TNF RI/TNFRSF1A Antibody (55R170)

Customer Reviews for Mouse TNF RI/TNFRSF1A Antibody (55R170) (2)

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  • Mouse TNF RI/TNFRSF1A Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Macrophages
    Species: Mouse
    Verified Customer | Posted 10/20/2021
    Mouse TNF RI/TNFRSF1A Antibody (55R170) MAB430
  • Mouse TNF RI/TNFRSF1A Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: purified IgG
    Species: Human
    Verified Customer | Posted 06/28/2016
    murine cross-reactivity studies

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