Mouse ULBP-1 /MULT-1 Antibody (237104)

R&D Systems | Catalog # MAB2588

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry, ELISA Development, Functional Assay

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 237104
View all ULBP-1 Primary Antibodies »

Product Specifications

Immunogen

BaF3 mouse pro-B cell line transfected with mouse ULBP‑1 /MULT-1

Specificity

Detects mouse ULBP‑1 /MULT-1.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse ULBP-1 /MULT-1 Antibody (237104)

Detection of ULBP‑1 in A20 Mouse Cell Line by Flow Cytometry.

A20 mouse B cell lymphoma cell line was stained with Rat Anti-Mouse ULBP-1 /MULT-1 Monoclonal Antibody (Catalog # MAB2588, filled histogram) or isotype control antibody (MAB006, open histogram) followed by anti-Rat IgG PE-conjugated secondary antibody (F0105B). View our protocol for Staining Membrane-associated Proteins.
Detection of ULBP-1/MULT-1 by Immunohistochemistry

Detection of ULBP-1/MULT-1 by Immunohistochemistry

NKG2DLs are overexpressed in hepatocellular carcinoma and correlate with poor prognosis. A Pan-cancer analysis of NKG2DL expression profiles across multiple tumor types from the TCGA database. B Differential expression of major NKG2DL subtypes between human HCC and matched adjacent non-tumorous liver tissues. C-D Kaplan-Meier overall survival curves of HCC patients stratified by high vs. low expression of ULBP2 (C) and ULBP1 (D). E NKG2DL expression levels across different HCC histological grades. F Survival analysis based on NKG2DL expression within distinct histological grade subgroups. G A nomogram integrating NKG2DL expression and clinical parameters for predicting 1-, 3-, and 5-year overall survival. H Calibration curves assessing the concordance between nomogram-predicted and observed survival probabilities. I Time-dependent receiver operating characteristic (ROC) analysis evaluating the diagnostic performance of NKG2DLs for predicting patient survival. J Representative IHC images of ULBP2 and ULBP1 in human HCC and adjacent non-tumorous tissues. K–N Quantitative IHC scores for ULBP2 (K) and ULBP1 (M) in paired tumor and non-tumor specimens, with their corresponding Kaplan-Meier survival analyses (L, N). O Flow cytometry analysis of NKG2DL surface expression on a panel of murine tumor cell lines. P Schematic diagram of the lentiviral construct designs for GFP-M (control) and NKG2D-targeting CAR-M. Q Efficiency of lentiviral transduction in iBMDM-derived macrophages, as indicated by EGFP reporter expression. R Surface expression levels of the engineered NKG2D-CAR on macrophages, confirmed by flow cytometry Image collected and cropped by CiteAb from the following open publication (https://link.springer.com/10.1186/s12943-025-02538-w), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse ULBP-1 /MULT-1 Antibody (237104)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: A20 mouse B cell lymphoma cell line

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ULBP-1

ULBP-1, also known as MULT-1 (mouse UL16-binding protein-like Transcript 1), is a 53 kDa, MHC Class I-like molecule that belongs to the mouse family of NKG2D ligands (1‑4). It is a type I transmembrane glycoprotein that is synthesized as a 334 amino acid (aa) precursor. It contains a 25 aa signal sequence, a 186 aa extracellular region, a 19 aa transmembrane segment and a 104 aa cytoplasmic tail (2). The extracellular region contains an alpha 1 and alpha 2 like domain with two intrachain disulfide bonds. ULBP-1 is distantly related to other human and mouse NKG2D ligands, and more distantly related to the MHC class I proteins (3). Unlike most NKG2D ligands, transcripts for ULBP-1 have been detected in a wide variety of mouse tissues and tumor cells lines (3). The receptor for ULBP-1 is NKG2D, a 35 kDa C-type lectin that is found on mouse NK cells, activated CD8+ T cells, epidermal gamma delta T cells, and activated macrophages (1, 5, 6, 7). Recombinant ULBP-1 protein binds to NKG2D with high affinity (KD = 6 nM) (2). Although an activating receptor, general cellular responses to NKG2D ligation depend upon the isoform of NKG2D and the cell type (5). Exposure to immobilized ULBP-1 or ULBP-1-transfected cells elicits IFN-gamma production by NK cells (3). Ectopic expression of ULBP-1 on the RMA mouse tumor cell line leads to tumor rejection in syngeneic mice (3).

References

  1. Raulet, D.H. (2003) Nat. Rev. Immunol. 3:781.
  2. Carayannopoulos, L. et al. (2002) J. Immunol. 169:4079.
  3. Diefenbach, A. et al. (2003) Eur. J. Immunol. 33:381.
  4. Krmpotic, A. et al. (2005) J. Exp. Med. 201:211.
  5. Diefenbach, A. et al. (2002) Nat. Immunol. 3:1142.
  6. Ho, E.L. et al. (1998) Proc. Natl. Acad. Sci. USA 95:6320.
  7. Carayannopoulos, L.N. et al. (2002) Eur. J. Immunol. 32:597.

Long Name

UL16 Binding Protein-1

Alternate Names

ALCAN-beta, MULT-1, NKG2DL, NKG2DL1, RAET1I, ULBP1

Entrez Gene IDs

80329 (Human); 77777 (Mouse)

Gene Symbol

ULBP1

Additional ULBP-1 Products

Product Documents for Mouse ULBP-1 /MULT-1 Antibody (237104)

Certificate of Analysis

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Product Specific Notices for Mouse ULBP-1 /MULT-1 Antibody (237104)

For research use only

Citations for Mouse ULBP-1 /MULT-1 Antibody (237104)

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