MPHOSPH1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-18516

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MPHOSPH1 Primary Antibodies »

Product Specifications

Immunogen

Recognizes region between residues Between Between 825 and 875..

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MPHOSPH1 Antibody - BSA Free

MPHOSPH1 Antibody

Immunoprecipitation;MPHOSPH1 Antibody[NBP3-18516]

Immunoprecipitation;MPHOSPH1 Antibody Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-KIF20B antibody used for IP at 6 µg per reaction. KIF20B was also immunoprecipitated by rabbit anti-KIF20B antibody. For bting immunoprecipitated KIF20B, was used at 0.4 mg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for MPHOSPH1 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

8 - 15 ug/mg lysate

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Tris-citrate/phosphate pH 7 -8.

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: MPHOSPH1

MPHOSPH1, also known as Kinesin-like protein KIF20B, has 4 isoforms, a 1,820 amino acid isoform that is 211 kDa, a 1,853 amino acid isoform that is 214 kDa, a 1,780 amino acid isoform that is 200 kDa, and a 512 amino acid isoform that is 60 kDa, localizes mainly in the nucleus during interphase although it is also detected in the cytoplasm without clear association with microtubules, it is found in brain, ovary, kidney and testis, and acts as a plus-end-directed motor enzyme that is required for completion of cytokinesis. Disease research is currently being studied with relation to MPHOSPH1 and myeloproliferative disorder, intrahepatic cholangiocarcinoma, cholangiocarcinoma, Alzheimer's disease, and ataxia. This protein involvement has been observed with trelation to MYC, PIN1, RUVBL1, YWHAB, and CDK1proteins in M phase of mitotic cell cycle, ATP catabolic process, microtubule-based movement, cell cycle arrest, and mitosis pathways.

Alternate Names

Cancer/testis antigen 90, CT90MPP-1, DKFZp434B0435, EC 6.3.5.5, kinesin family member 20B, Kinesin-related motor interacting with PIN1, KRMP1kinesin-like protein KIF20B, M-phase phosphoprotein 1mitotic kinesin-like protein, MPHOSPH1EC 2.1.1, MPP1DKFZp434P0810

Gene Symbol

KIF20B

Additional MPHOSPH1 Products

Product Documents for MPHOSPH1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for MPHOSPH1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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