MxA/Mx1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-32905

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Bovine, Monkey, Rabbit

Predicted:

Chimpanzee (98%), Rhesus Macaque (92%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MxA/Mx1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human MxA/Mx1. The exact sequence is proprietary.

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

76 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MxA/Mx1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: MxA/Mx1 Antibody [NBP1-32905]

Immunocytochemistry/ Immunofluorescence: MxA/Mx1 Antibody [NBP1-32905]

Immunocytochemistry/Immunofluorescence: MxA/Mx1 Antibody [NBP1-32905] - Detects MX1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MX1 protein stained by MX1 antibody, Internal diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:1000. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: MxA/Mx1 Antibody [NBP1-32905]

Immunohistochemistry-Paraffin: MxA/Mx1 Antibody [NBP1-32905]

Immunohistochemistry-Paraffin: MxA/Mx1 Antibody [NBP1-32905] - Human lymph node. Antigen retrieval in citrate buffer, 95C for 30 min. Image submitted by a verified customer review.
Western Blot: MxA/Mx1 Antibody [NBP1-32905]

Western Blot: MxA/Mx1 Antibody [NBP1-32905]

Western Blot: MxA/Mx1 Antibody [NBP1-32905] - A. 30 ug 293T whole cell lysate/extract B. 30 ug whole cell lysate/extract of human MX1-transfected 293T cells. 7.5 % SDS-PAGE MX1 antibody [N2C2], Internal dilution: 1:10000
Immunohistochemistry-Paraffin: MxA/Mx1 Antibody [NBP1-32905]

Immunohistochemistry-Paraffin: MxA/Mx1 Antibody [NBP1-32905]

Immunohistochemistry-Paraffin: MxA/Mx1 Antibody [NBP1-32905] - MDA-MB-468 xenograft, using MX1antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: MxA/Mx1 Antibody [NBP1-32905]

Immunohistochemistry-Paraffin: MxA/Mx1 Antibody [NBP1-32905]

Immunohistochemistry-Paraffin: MxA/Mx1 Antibody [NBP1-32905] - Human colon carcinoma. MX1 antibody [N2C2], Internal diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
MxA/Mx1 Antibody - BSA Free

Western Blot: MxA/Mx1 Antibody [NBP1-32905] -

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MX1 antibody [N2C2], Internal (NBP1-32905) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
MxA/Mx1 Antibody - BSA Free

Flow Cytometry: MxA/Mx1 Antibody - BSA Free [NBP1-32905] -

The correlation between miR-141 level and the expression profile of MxA and STAT3 in infected A549 cells. (A) Quantification of steady-state miR-141 in infected A549 cells with MOI of 0.5 and transfected with either pre-miR-141 or miR-141 inhibitor compared with noninfected cells (control) using qRT-PCR. (B) Relative gene expression of MxA and STAT3 in infected A549 cells transfected with either specific inhibitor against miR-141 or pre- miR-141 compared with control-transfected cells using qRT-PCR. Error bars indicate the STD of three independent experiments. Student two-tailed t-test used for statistical analysis, (*) indicates P-values ≤ 0.05, and (**) indicates P ≤ 0.01. (C) Flow cytometric assay quantifies the kinetic proteins expression profile of MxA (in blue dots) and STAT3 (in red dots) in infected and transfected A549 cells compared with control cells. (D) Western blot analysis reveals the protein expression level of MxA and STAT3 in infected and transfected cells compared to control cells, beta -actin expression profile severed as an internal control Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37596622), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
MxA/Mx1 Antibody - BSA Free

Flow Cytometry: MxA/Mx1 Antibody - BSA Free [NBP1-32905] -

The correlation between miR-141 level and the expression profile of MxA and STAT3 in infected A549 cells. (A) Quantification of steady-state miR-141 in infected A549 cells with MOI of 0.5 and transfected with either pre-miR-141 or miR-141 inhibitor compared with noninfected cells (control) using qRT-PCR. (B) Relative gene expression of MxA and STAT3 in infected A549 cells transfected with either specific inhibitor against miR-141 or pre- miR-141 compared with control-transfected cells using qRT-PCR. Error bars indicate the STD of three independent experiments. Student two-tailed t-test used for statistical analysis, (*) indicates P-values ≤ 0.05, and (**) indicates P ≤ 0.01. (C) Flow cytometric assay quantifies the kinetic proteins expression profile of MxA (in blue dots) and STAT3 (in red dots) in infected and transfected A549 cells compared with control cells. (D) Western blot analysis reveals the protein expression level of MxA and STAT3 in infected and transfected cells compared to control cells, beta -actin expression profile severed as an internal control Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37596622), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for MxA/Mx1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

Assay dependent

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Reviewed Applications

Read 1 review rated 4 using NBP1-32905 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MxA/Mx1

In mouse, the interferon-inducible Mx protein is responsible for a specific antiviral state against influenza virus infection. The protein encoded by this gene is similar to the mouse protein as determined by its antigenic relatedness, induction conditions, physicochemical properties, and amino acid analysis. This cytoplasmic protein is a member of both the dynamin family and the family of large GTPases. [provided by RefSeq]

Long Name

Interferon-regulated Resistance GTP-binding protein MxA

Alternate Names

IFI-78K, IFI78, Mx1

Gene Symbol

MX1

Additional MxA/Mx1 Products

Product Documents for MxA/Mx1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for MxA/Mx1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Related Research Areas

Infectious Diseases

Citations for MxA/Mx1 Antibody - BSA Free

Customer Reviews for MxA/Mx1 Antibody - BSA Free (1)

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  • MxA/Mx1 Antibody
    Name: Santhosh Sivajothi
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Human lymph node
    Species: Human
    Verified Customer | Posted 09/25/2018
    Heat mediated antigen retrieval performed in citrate buffer for 30 mins at 95C
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Protocols

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FAQs

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