Myc Epitope Tag Antibody (2) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-43627
![Western Blot: Myc Epitope Tag Antibody (2) [NBP2-43627]](https://resources.rndsystems.com/images/products/Myc-Epitope-Tag-Antibody-2-Western-Blot-NBP2-43627-img0004.jpg "Western Blot: Myc Epitope Tag Antibody (2) [NBP2-43627]")
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Key Product Details
Species Reactivity
Epitope Tag
Applications
Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 2
Format
Azide and BSA Free
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Product Specifications
Immunogen
The immunogen used to generate this antibody corresponds to Myc Epitope Tag
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Myc Epitope Tag Antibody (2) - Azide and BSA Free
Western Blot: Myc Epitope Tag Antibody (2) [NBP2-43627]
Western Blot: Myc Epitope Tag Antibody (2) [NBP2-43627] - Myc tag antibody [0002] Immunoprecipitation of Myc tagged protein. HeLa transfected lysates were subjected to immunoprecipitation using (B) normal mouse IgG or (C) 2.5 ug of anti-Myc tag antibody [0002]. (A) Input, 20ug of HeLa transfected lysates. The precipitated protein was detected. EasyBlot anti-Mouse IgG Kit was used in Western blot.![Immunocytochemistry/ Immunofluorescence: Myc Epitope Tag Antibody (2) [NBP2-43627]](https://resources.rndsystems.com/images/products/Myc-Epitope-Tag-Antibody-2-Immunocytochemistry-Immunofluorescence-NBP2-43627-img0006.jpg "Immunocytochemistry/ Immunofluorescence: Myc Epitope Tag Antibody (2) [NBP2-43627]")
Immunocytochemistry/ Immunofluorescence: Myc Epitope Tag Antibody (2) [NBP2-43627]
Immunocytochemistry/Immunofluorescence: Myc Epitope Tag Antibody (2) [NBP2-43627] - Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Myc tag stained by Myc tag antibody [0002] (NBP2-43627) diluted at 1:2000.
Western Blot: Myc Epitope Tag Antibody (2) [NBP2-43627] -
Western Blot: Myc Epitope Tag Antibody (2) [NBP2-43627] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Myc Epitope Tag antibody [GT0002] (NBP2-43627) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
ELISA: Myc Epitope Tag Antibody (2) [NBP2-43627] -
Indirect ELISA analysis was performed by coating the plate with recombinant Multiple Tags Positive Control ( 6xHis-MBP-DDDDK-V5-HSV-HA-T7-Myc-S) (57.14-0.89 nM). Coated protein was probed with Myc tag antibody [GT0002] (NBP2-43627) (1 ug/mL). Goat antibody Mouse IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.Applications for Myc Epitope Tag Antibody (2) - Azide and BSA Free
Application
Recommended Usage
ELISA
Assay dependent
Immunocytochemistry/ Immunofluorescence
1:100-1:2000
Immunoprecipitation
1:100-1:500
Western Blot
1:5000-1:20000
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Myc Epitope Tag
Alternate Names
BHLHE39, Class E basic helix-loop-helix protein 39 (bHLHe39), Myc proto-oncogene protein, Proto-oncogene c-Myc, Transcription factor p64
Additional Myc Epitope Tag Products
Product Documents for Myc Epitope Tag Antibody (2) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Myc Epitope Tag Antibody (2) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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