Myosin 5a Antibody

Name: Myosin 5a Antibody
Brand: Novus Biologicals
SKU: NBP1-49858
Price: 399 USD
Availability: InStock

Novus Biologicals | Catalog # NBP1-49858

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

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Product Specifications

Immunogen

Peptide with sequence C-ETKQLELDLN corresponding to internal region according to NP_001135967.1.

Epitope

ETKQLELDLN - internal region

Specificity

This antibody is expected to recognize both reported isoforms (NP_000250.3; NP_001135967.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Myosin 5a Antibody

Western Blot: Myosin 5a Antibody [NBP1-49858]

Western Blot: Myosin 5a Antibody [NBP1-49858] - Analysis of Myosin 5a in Jurkat lysate (35ug protein in RIPA buffer) using this antibody at 1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Immunocytochemistry/ Immunofluorescence: Myosin 5a Antibody [NBP1-49858]

Immunocytochemistry/Immunofluorescence: Myosin 5a Antibody [NBP1-49858] - (1ug/ml) staining of Mouse Skeletal Muscle (first panel, and in green in third panel). Alpha-bungaratoxin staining in middle panel and in red in third panel. Detected by Fluorescence. Data kindly provided by Dr. Rudiger Rudolf, Karlsruhe, Germany

Applications for Myosin 5a Antibody

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1 - 3 ug/ml

Peptide ELISA

Detection limit 1:8000

Western Blot

1 - 3 ug/ml

Application Notes

IF: Transverse sections of mouse Skeletal Muscle shows staining of the fibre membrane and neuromuscular junction. The observed pattern corresponds to earlier findings in literature with different antibodiesscientific literature (PMID: 20133847). WB: Approx. 200 kDa band observed in Jurkat lysates (calculated MW of 215 kDa band according to NP_000250.3).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Myosin 5a

The MYO5 gene is one of three myosin V heavy-chain genes, belonging to the myosin gene superfamily. Myosin V is a class of actin-based motor proteins involved in cytoplasmic vesicle transport and anchorage, spindle-pole alignment and mRNA translocation. The p

Alternate Names

Dilute myosin heavy chain, non-muscle, GS1myosin, heavy polypeptide kinase, MYH12myosin-12, MYO5, Myosin heavy chain 12, myosin V, myosin VA (heavy chain 12, myoxin), myosin VA (heavy polypeptide 12, myoxin), Myosin-12, myosin-Va, myoxin, MYR12

Entrez Gene IDs

4644 (Human)

Gene Symbol

MYO5A

UniProt

NP_000250.3 (Human)

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Product Specific Notices for Myosin 5a Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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