Myosin Phosphatase [p Thr850, p Thr853] Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-13185

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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat, Chicken

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Myosin Phosphatase Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to aa845-855, C-EKRRS[pT]GVSFW of chicken MYPT1 with an N-terminal cysteine added for conjugation purposes. Phosphorylation site corresponds to Thr853 in human MYPT1.

Reactivity Notes

Crossreactivity: human (Thr853, O014974), chicken (Thr850, Q90623), mouse (Thr852, Q9DBR7) and rat (Thr855, Q10728).

Modification

p Thr853, p Thr850

Specificity

Recognizes recombinant truncated MYPT1 phosphorylated on Thr853, MW ~80kDa

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Scientific Data Images for Myosin Phosphatase [p Thr850, p Thr853] Antibody - BSA Free

Myosin Phosphatase [p Thr853] Antibody [NBP2-13185] - Lane 1: untreated C2C12 lysate| Lane 2: pervanadate treated C2C12 lysate. Resolved by electrophoresis, transferred to nitrocellulose and probed with Myosin Phosphatase [p Thr853] antibody (0.5ug/ml). Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system. Arrow indicates phosphorylated MYPT1 substrate (80kD).
Myosin Phosphatase [p Thr853] Antibody [NBP2-13185] - Lane 1: ROKa/ROCK-II only Lane 2: MYPT1 substrate only Lane 3: MYPT1 substrate posphorylated by ROKa/ROCK-II 3

Applications for Myosin Phosphatase [p Thr850, p Thr853] Antibody - BSA Free

Application
Recommended Usage

Western Blot

0.5-2 ug/ml
Application Notes
Western Blot: Detects MYPT1 by preincubation with ROKa/ROK-II active or C2C12 lysates treated with pervanadate. Optimal dilutions to be determined by the researcher.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.1M Tris-Glycine (pH 7.4), 0.15M NaCl and 30% Glycerol

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Myosin Phosphatase

Myosin phosphatase target subunit 1 (MYPT1), which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho, as previously shown (6). To understand the role of MYPT1 in the regulation of the cytoskeleton in human diseases, a 4855-bp cDNA was cloned for human MYPT1 using the rat MYPT1 cDNA as probe. Sequencing analysis has revealed that human MYPT1 contains 1030 amino acid residues with a calculated molecular mass of approximately 115kD. Fluorescence in situ hybridization analysis placed the human MYPT1 gene on chromosome 12q15-q21.2. Radiation hybrid mapping has shown that the human MYPT1 gene is located very near the highly polymorphic marker CHLC.GATA65A12, which lies between D12S350 and D12S106.

Alternate Names

MBSmyosin phosphatase, target subunit 1, MGC133042, Myosin phosphatase target subunit 1, Myosin phosphatase-targeting subunit 1, MYPT1protein phosphatase 1 regulatory subunit 12A, protein phosphatase 1, regulatory (inhibitor) subunit 12A, Protein phosphatase myosin-binding subunit

Gene Symbol

PPP1R12A

UniProt

Q90623 (Chicken)

Additional Myosin Phosphatase Products

Product Documents for Myosin Phosphatase [p Thr850, p Thr853] Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Myosin Phosphatase [p Thr850, p Thr853] Antibody - BSA Free

May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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