N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-32899

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (91%), Canine (92%), Porcine (91%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all N-Acetylgalactosamine-6-Sulfatase/GALNS Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human N-Acetylgalactosamine-6-Sulfatase/GALNS. The exact sequence is proprietary.

Reactivity Notes

Zebrafish (81%), Xenopus laevis (84%), Xenopus Tropicalis (80%).

Localization

Lysosome

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody [NBP1-32899]

Immunocytochemistry/ Immunofluorescence: N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody [NBP1-32899]

Immunocytochemistry/Immunofluorescence: N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody [NBP1-32899] - Paraformaldehyde-fixed HeLa, using GALNS antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled Red.
Immunohistochemistry-Paraffin: N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody [NBP1-32899]

Immunohistochemistry-Paraffin: N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody [NBP1-32899]

Immunohistochemistry-Paraffin: N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody [NBP1-32899] - Paraffin-embedded NCI-N87 xenograft, using antibody at 1:100 dilution.
Western Blot: N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody [NBP1-32899]

Western Blot: N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody [NBP1-32899]

Western Blot: N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody [NBP1-32899] - Sample (30 ug of whole cell lysate) A: H1299 7.5% SDS PAGE diluted at 1:1000.
N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody - BSA Free

Western Blot: N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody [NBP1-32899] -

N-Acetylgalactosamine-6-Sulfatase/GALNS antibody detects N-Acetylgalactosamine-6-Sulfatase/GALNS protein by western blot analysis.
A. 30 ug PC-12 whole cell lysate/extract
B. 30 ug Rat2 whole cell lysate/extract
7.5% SDS-PAGE
N-Acetylgalactosamine-6-Sulfatase/GALNS antibody (NBP1-32899) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody - BSA Free

Western Blot: N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody [NBP1-32899] -

GALNS antibody detects GALNS protein by western blot analysis.
A. 30 ug PC-12 whole cell lysate/extract
B. 30 ug Rat2 whole cell lysate/extract
7.5% SDS-PAGE
GALNS antibody (NBP1-32899) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody - BSA Free

Western Blot: N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody [NBP1-32899] -

GALNS antibody detects GALNS protein by western blot analysis.
A. 30 ug Neuro2A whole cell lysate/extract
B. 30 ug C2C12 whole cell lysate/extract
7.5% SDS-PAGE
GALNS antibody (NBP1-32899) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Reviewed Applications

Read 2 reviews rated 4 using NBP1-32899 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: N-Acetylgalactosamine-6-Sulfatase/GALNS

This gene encodes N-acetylgalactosamine-6-sulfatase which is a lysosomal exohydrolase required for the degradation of the glycosaminoglycans, keratan sulfate, and chondroitin 6-sulfate. Sequence alterations including point, missense and nonsense mutations, as well as those that affect splicing, result in a deficiency of this enzyme. Deficiencies of this enzyme lead to Morquio A syndrome, a lysosomal storage disorder. [provided by RefSeq]

Alternate Names

Chondroitinase, Chondroitinsulfatase, GA6S, Galactose-6-Sulfate Sulfatase, GalNAc6S Sulfatase, GALNS, GAS, MPS4A, NAcetylgalactosamine6Sulfatase

Entrez Gene IDs

2588 (Human)

Gene Symbol

GALNS

Additional N-Acetylgalactosamine-6-Sulfatase/GALNS Products

Product Documents for N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Customer Reviews for N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody - BSA Free (2)

4 out of 5
2 Customer Ratings
5 Stars
0%
4 Stars
100%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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  • Name: Yadilette Rivera-Colon
    Application: Western Blot
    Sample Tested: Human Protein transfected in Tn5 cells
    Species: Human
    Verified Customer | Posted 07/08/2010
  • Name: Yadilette Rivera-Colon
    Application: Western Blot
    Sample Tested: Tn5 Cell supernatant
    Species: Human
    Verified Customer | Posted 06/24/2010

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Protocols

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