NanoLuc® (Nluc) Luciferase Antibody (965808)

R&D Systems | Catalog # MAB10026

R&D Systems

Key Product Details

Species Reactivity

Validated:

Multi-Species

Cited:

Human, Mouse, Canine, Fish - Danio rerio (Zebrafish), Primate - Chlorocebus aethiops (African Green Monkey)

Applications

Validated:

Western Blot

Cited:

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 965808
View all Luciferase Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide of NanoLuc® (Nluc) Luciferase

Specificity

Detects NanoLuc® (Nluc) Luciferase in direct ELISAs. Detects NanoLuc® (Nluc) Luciferase and Large BiT in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for NanoLuc® (Nluc) Luciferase Antibody (965808)

Detection of NanoLuc® Luciferase and Large BiT antibody by Western Blot.

Detection of NanoLuc® Luciferase and Large BiT by Western Blot.

Western blot shows Recombinant Large BiT and Recombinant NanoLuc®. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human NanoLuc® (Nluc) Luciferase Monoclonal Antibody (Catalog # MAB10026) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for NanoLuc® and Large BiT at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for NanoLuc® (Nluc) Luciferase Antibody (965808)

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Recombinant Large BiT and Recombinant NanoLuc®

Reviewed Applications

Read 2 reviews rated 4.5 using MAB10026 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Luciferase

NanoLuc® (Nluc) luciferase is a small enzyme (19.1kDa) engineered for optimal performance as a luminescent reporter. NanoLuc® Binary Technology (NanoBiT™) is a two subunit system based on NanoLuc® luciferase. Large BiT (LgBiT; 17.6kDa) is the large subunit of the NanoBiT® system.

References


Alternate Names

LuC, luciferin 4 monooxygenase, Luciferin 4-monooxygenase

Additional Luciferase Products

Product Documents for NanoLuc® (Nluc) Luciferase Antibody (965808)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for NanoLuc® (Nluc) Luciferase Antibody (965808)

NanoLuc and NanoBiT are registered trademarks of Promega Corporation.

For research use only

Citations for NanoLuc® (Nluc) Luciferase Antibody (965808)

Customer Reviews for NanoLuc® (Nluc) Luciferase Antibody (965808) (2)

4.5 out of 5
2 Customer Ratings
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Customer Images

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  • NanoLuc® (Nluc) Luciferase Antibody
    Name: Franziska Gsottberger
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: HEK293T human embryonic kidney cell line
    Species: Human
    Verified Customer | Posted 04/28/2020
    NanoLuc® (Nluc) Luciferase Antibody (965808) MAB10026
  • NanoLuc® (Nluc) Luciferase Antibody
    Name: Juan Guzman
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: HEK293 human embryonic kidney cell line
    Species: Human
    Verified Customer | Posted 04/23/2020
    NanoLuc® (Nluc) Luciferase Antibody (965808) MAB10026

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FAQs for NanoLuc® (Nluc) Luciferase Antibody (965808)

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  • Q: What is the immunogen for the NanoLuc® (Nluc) Luciferase Antibody, Catalog # MAB10026? Does this antibody recognize only LgBiT and not SmBiT?

    A: The immunoogen for Catalog # MAB10026 is Synthetic peptide of NanoLuc® (Nluc) Luciferase. For more details on the compound, Promega Corporation should be contacted. NanoBit (LgBit+SmBit) is a derivative of the original product NanoLuc,  where portion of the protein is split to get two complementary peptides. This antibody detects LgBiT and not SmBiT.

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