Nav1.7 Antibody

Novus Biologicals | Catalog # NBP3-48639

Novus Biologicals
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Key Product Details

Species Reactivity

Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Nav1.7 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of mouse Nav1.7. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Nav1.7 Antibody

Nav1.7 Antibody

Western Blot: Nav1.7 Antibody [NBP3-48639] -

Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Nav1.7 antibody (NBP3-48639) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Nav1.7 Antibody

Immunocytochemistry/ Immunofluorescence: Nav1.7 Antibody [NBP3-48639] -

Nav1.7 antibody detects Nav1.7 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Nav1.7 stained by Nav1.7 antibody (NBP3-48639) diluted at 1:500.
Red: Tau, stained by Tau antibody [GT287] diluted at 1:500.
Blue: Fluoroshield with DAPI.
Nav1.7 Antibody

Western Blot: Nav1.7 Antibody [NBP3-48639] -

Various tissue extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Nav1.7 antibody (NBP3-48639) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Nav1.7 Antibody

Immunocytochemistry/ Immunofluorescence: Nav1.7 Antibody [NBP3-48639] -

Nav1.7 antibody detects Nav1.7 protein at cell membrane by immunofluorescent analysis.
Sample: mouse dorsal root ganglia cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Nav1.7 stained by Nav1.7 antibody (NBP3-48639) diluted at 1:500.
Red: NF-H, an axon marker, stained by NF-H antibody [GT114] diluted at 1:500.
Blue: Fluoroshield with DAPI.

Applications for Nav1.7 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nav1.7

Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient. It is a tetrodotoxin-sensitive Na(+) channel isoform. Plays a role in pain mechanisms, especially in the development of inflammatory pain (from SwissProt).

Long Name

Sodium channel protein type 9 subunit alpha

Alternate Names

hNE-Na, Ne-Na, NENA, PN1, SCN9A

Gene Symbol

SCN9A

Additional Nav1.7 Products

Product Documents for Nav1.7 Antibody

Certificate of Analysis

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Product Specific Notices for Nav1.7 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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