NCAPD3 Antibody
Novus Biologicals | Catalog # NB100-1573
![Western Blot: NCAPD3 Antibody [NB100-1573]](https://resources.rndsystems.com/images/products/NCAPD3-Antibody-NB100-1573-img0001.jpg "Western Blot: NCAPD3 Antibody [NB100-1573]")
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Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Cited:
Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 1450 and the C-terminus (residue 1507) of human Chromosome-Associated Protein D3 using the numbering given in entry NP_056076.1 (GeneID 23310).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for NCAPD3 Antibody
Western Blot: NCAPD3 Antibody [NB100-1573]
Western Blot: NCAPD3 Antibody [NB100-1573] - Detection of human CAP-D3 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CAP-D3 antibody NB100-1573 (lotNB100-1573-4) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.![Immunoprecipitation: NCAPD3 Antibody [NB100-1573]](https://resources.rndsystems.com/images/products/NCAPD3-Antibody-NB100-1573-img0002.jpg "Immunoprecipitation: NCAPD3 Antibody [NB100-1573]")
Immunoprecipitation: NCAPD3 Antibody [NB100-1573]
Immunoprecipitation: NCAPD3 Antibody [NB100-1573] - Detection of human CAP-D3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CAP-D3 antibody (lot NB100-1573-4) used for IP at 6 ug per reaction. CAP-D3 was also immunoprecipitated by a previous lot of this antibody (lot NB100-1573-3). For blotting immunoprecipitated CAP-D3, NB100-1573 was used at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.Applications for NCAPD3 Antibody
Application
Recommended Usage
Immunoprecipitation
2-10 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Use in ICC/IF reported in scientific literature (PMID: 30455981).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: NCAPD3
Alternate Names
CAPD3, CAP-D3, FLJ42888, hCAP-D3condensin-2 complex subunit D3, hcp-6, hHCP-6, KIAA0056MGC104671, Non-SMC condensin II complex subunit D3, non-SMC condensin II complex, subunit D3
Entrez Gene IDs
23310 (Human)
Gene Symbol
NCAPD3
UniProt
Additional NCAPD3 Products
Product Documents for NCAPD3 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for NCAPD3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for NCAPD3 Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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