NCAPG2 Antibody

Novus Biologicals | Catalog # NB100-1813

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all NCAPG2 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1100 and the C-terminus (residue 1143) of human Chromosome-Associated Protein G2 using the numbering given in entry AAH43404.1 (GeneID 54892).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

131 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NCAPG2 Antibody

Western Blot: NCAPG2 Antibody [NB100-1813]

Western Blot: NCAPG2 Antibody [NB100-1813]

Western Blot: NCAPG2 Antibody [NB100-1813] - Detection of Human CAP-G2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CAP-G2 antibody NB100-1813 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: NCAPG2 Antibody [NB100-1813]

Immunoprecipitation: NCAPG2 Antibody [NB100-1813]

Immunoprecipitation: NCAPG2 Antibody [NB100-1813] - Detection of human CAP-G2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CAP-G2 antibody NB100-1813 used for IP at 6 ug per reaction. CAP-G2 was also immunoprecipitated by another rabbit anti-CAP-G2 antibody. For blotting immunoprecipitated CAP-G2, NB100-1813 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for NCAPG2 Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg of lysate

Western Blot

1:2000-1:10000
Application Notes
Use in ICC/IF reported in scientific literature (PMID: 30143891). Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: NCAPG2

CAP-G2 is a subunit of the heteropentameric condensin II complex. Condensin participates in the condensation of chromosomes during cell division by facilitating positive DNA supercoiling and knotting by topoisomerase I and II and proper segregation of sister chromatids. CAP-G2 is a non-SMC regulatory subunit of the condensin complex. Alternate names for CAP-G include condensin-II complex subunit G2, chromosome-associated protein G2, hCAP-G2, CAP-G2, Non-SMC condensin II complex subunit G2, leucine zipper protein 5, NCAPG2, LUZP5, MTB, and FLJ20311.

Alternate Names

Chromosome-associated protein G2, FLJ20311, Leucine zipper protein 5CAP-G2hCAP-G2condensin-2 complex subunit G2, LUZP5, more than blood homolog, MTB, Non-SMC condensin II complex subunit G2, non-SMC condensin II complex, subunit G2

Entrez Gene IDs

54892 (Human)

Gene Symbol

NCAPG2

UniProt

Q86XI2 (Human)

Additional NCAPG2 Products

Product Documents for NCAPG2 Antibody

Certificate of Analysis

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Product Specific Notices for NCAPG2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for NCAPG2 Antibody

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Protocols

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