NCAPH2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-28723

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all NCAPH2 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 425 and 475 of human chromosome-associated protein H2 using the numbering given in entry NP_689512.2 (GeneID 29781).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NCAPH2 Antibody - BSA Free

Western Blot: NCAPH2 Antibody [NBP1-28723]

Western Blot: NCAPH2 Antibody [NBP1-28723]

Western Blot: NCAPH2 Antibody [NBP1-28723] - Detection of Human CapH2 by Western Blot. Samples: Whole cell lysate (15 and 50 ug) from HeLa and (50 ug) from 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CapH2 antibody NBP1-28723 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry: NCAPH2 Antibody [NBP1-28723]

Immunohistochemistry: NCAPH2 Antibody [NBP1-28723]

Immunohistochemistry: NCAPH2 Antibody [NBP1-28723] - Detection of human CAP-H2 by immunohistochemistry. Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti-CAP-H2 (NBP1-28723). Detection: DAB
Western Blot: NCAPH2 Antibody [NBP1-28723]

Western Blot: NCAPH2 Antibody [NBP1-28723]

Western Blot: NCAPH2 Antibody [NBP1-28723] - Detection of Human CAP-H2 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-CAP-H2 antibody NBP1-28723 used for WB at 1 mcg/ml (A and B) and used for IP at 10 mcg/mg lysate. CAP-H2 was also immunoprecipitated by rabbit anti-CAP-H2 antibody NBP1-28682, which recognizes a downstream epitope. For blotting immunoprecipitated CapH2, the ReliaBLOT Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).
Immunohistochemistry-Paraffin: NCAPH2 Antibody [NBP1-28723]

Immunohistochemistry-Paraffin: NCAPH2 Antibody [NBP1-28723]

Immunohistochemistry-Paraffin: NCAPH2 Antibody [NBP1-28723] - FFPE section of human skin carcinoma. Antibody used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit.
Immunohistochemistry: NCAPH2 Antibody [NBP1-28723]

Immunohistochemistry: NCAPH2 Antibody [NBP1-28723]

Immunohistochemistry: NCAPH2 Antibody [NBP1-28723] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CAP-H2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit.
Immunoprecipitation: NCAPH2 Antibody [NBP1-28723]

Immunoprecipitation: NCAPH2 Antibody [NBP1-28723]

Immunoprecipitation: NCAPH2 Antibody [NBP1-28723] - Detection of human CapH2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CapH2 antibody NBP1-28723 used for IP at 6 ug per reaction. CapH2 was also immunoprecipitated by rabbit anti-CapH2 antibody NBP1-28682. For blotting immunoprecipitated CapH2, NBP1-28723 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for NCAPH2 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

10 ug/mg lysate

Western Blot

1:1000-1:5000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: NCAPH2

CAP-H2 (chromosome-associated protein H2) is 1 of 3 non-SMC subunits that define the condensin II complex. Condensin II along with the condensin I plays a critical role in mitotic chromosome assembly and segregation.

Alternate Names

CAP-H2, CAP-H2 subunit of the condensin II complex, CAPH2,384D8-2, Chromosome-associated protein H2, condensin-2 complex subunit H2, hCAP-H2, kleisin beta, Kleisin-beta, MGC15858, MGC18000, MGC2455, MGC4133, MGC5305, MGC8640, Non-SMC condensin II complex subunit H2, non-SMC condensin II complex, subunit H2

Entrez Gene IDs

29781 (Human)

Gene Symbol

NCAPH2

UniProt

NP_689512.2 (Human)

Additional NCAPH2 Products

Product Documents for NCAPH2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for NCAPH2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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