Nestin Antibody (CL0197) - BSA Free
Novus Biologicals | Catalog # NBP2-59775
![Western Blot: Nestin Antibody (CL0197) [NBP2-59775]](https://resources.rndsystems.com/images/products/Nestin-Antibody-CL0197-Western-Blot-NBP2-59775-img0004.jpg "Western Blot: Nestin Antibody (CL0197) [NBP2-59775]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CL0197
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed using a recombinant protein derived from P48681, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Nestin Antibody (CL0197) - BSA Free
Western Blot: Nestin Antibody (CL0197) [NBP2-59775]
Western Blot: Nestin Antibody (CL0197) [NBP2-59775] - Lane 1: Marker [kDa] Lane 2: Human cell line U-251 MG![Immunocytochemistry/ Immunofluorescence: Nestin Antibody (CL0197) [NBP2-59775]](https://resources.rndsystems.com/images/products/Nestin-Antibody-CL0197-Immunocytochemistry-Immunofluorescence-NBP2-59775-img0005.jpg "Immunocytochemistry/ Immunofluorescence: Nestin Antibody (CL0197) [NBP2-59775]")
Immunocytochemistry/ Immunofluorescence: Nestin Antibody (CL0197) [NBP2-59775]
Immunocytochemistry/Immunofluorescence: Nestin Antibody (CL0197) [NBP2-59775] - Staining of RH-30 cells using the Anti-NES monoclonal antibody, showing specific staining in intermediate filaments in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).![Immunohistochemistry-Paraffin: Nestin Antibody (CL0197) [NBP2-59775]](https://resources.rndsystems.com/images/products/Nestin-Antibody-CL0197-Immunohistochemistry-Paraffin-NBP2-59775-img0002.jpg "Immunohistochemistry-Paraffin: Nestin Antibody (CL0197) [NBP2-59775]")
Immunohistochemistry-Paraffin: Nestin Antibody (CL0197) [NBP2-59775]
Immunohistochemistry-Paraffin: Nestin Antibody (CL0197) [NBP2-59775] - Staining of human kidney shows strong positivity in renal glomeruli.![Western Blot: Nestin Antibody (CL0197) [NBP2-59775]](https://resources.rndsystems.com/images/products/Nestin-Antibody-CL0197-Western-Blot-NBP2-59775-img0003.jpg "Western Blot: Nestin Antibody (CL0197) [NBP2-59775]")
Western Blot: Nestin Antibody (CL0197) [NBP2-59775]
Western Blot: Nestin Antibody (CL0197) [NBP2-59775] - Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-NES monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.![Immunohistochemistry-Paraffin: Nestin Antibody (CL0197) [NBP2-59775]](https://resources.rndsystems.com/images/products/Nestin-Antibody-CL0197-Immunohistochemistry-Paraffin-NBP2-59775-img0001.jpg "Immunohistochemistry-Paraffin: Nestin Antibody (CL0197) [NBP2-59775]")
Immunohistochemistry-Paraffin: Nestin Antibody (CL0197) [NBP2-59775]
Immunohistochemistry-Paraffin: Nestin Antibody (CL0197) [NBP2-59775] - Staining of human cerebral cortex shows strong immunoreactivity in the endothelial cells.Applications for Nestin Antibody (CL0197) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry
1:2500 - 1:5000
Immunohistochemistry-Paraffin
1:2500 - 1:5000
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Nestin
Although it is often used as a marker for proliferating and migrating cells very little is known about Nestin's function or regulation.
Alternate Names
NES
Gene Symbol
NES
Additional Nestin Products
Product Documents for Nestin Antibody (CL0197) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Nestin Antibody (CL0197) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
