Neurogenin-2 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-98917

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all Neurogenin-2 Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human Neurogenin-2.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Neurogenin-2 Antibody - Azide and BSA Free

Western Blot: Neurogenin-2 Antibody [NBP2-98917]

Western Blot: Neurogenin-2 Antibody [NBP2-98917]

Western Blot: Neurogenin-2 Antibody [NBP2-98917] - Lane A: HepG2 Whole Cell Lysate Lane B: SH-SY5Y Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique.Performed under reducing conditions. Predicted band size:29 kDaObserved band size:29 kDa (We are unsure as to the identity of these extra bands.)
Immunoprecipitation: Neurogenin-2 Antibody [NBP2-98917]

Immunoprecipitation: Neurogenin-2 Antibody [NBP2-98917]

Immunoprecipitation: Neurogenin-2 Antibody [NBP2-98917] - Lane A:0.5 mg K562 Whole Cell Lysate 4 uL anti-NEUROG rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-NEUROG rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 26 kDa Observed band size: 26 kDa
Neurogenin-2 Antibody - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Neurogenin-2 Antibody [NBP2-98917] -

Immunocytochemistry/Immunofluorescence: Neurogenin-2 Antibody [NBP2-98917] - Immunofluorescence staining of Neurogenin-2 in HESS9 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98917 antibody (dilution ratio 1:300) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to nucleus.

Applications for Neurogenin-2 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunoprecipitation

4-8 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Neurogenin-2

Neurogenin-2 is a member of the neurogenin subfamily of basic helix-loop-helix (bHLH) transcription factor genes that play an important role in neurogenesis from migratory neural crest cells.[supplied by OMIM]

Alternate Names

Atoh4, Math4A, NEUROG2, Neurogenin2

Gene Symbol

NEUROG2

Additional Neurogenin-2 Products

Product Documents for Neurogenin-2 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Neurogenin-2 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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