Neuron-specific beta-III Tubulin Antibody (TuJ-1) [NL557]

R&D Systems | Catalog # NL1195R

R&D Systems

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Multi-Species

Cited:

Human, Mouse, Rabbit, Transgenic Mouse

Applications

Validated:

Immunocytochemistry

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Flow Cytometry

Label

NL557 (Excitation = 557 nm, Emission = 574 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # TuJ-1
View all beta-III Tubulin Primary Antibodies »

Product Specifications

Immunogen

Rat brain-derived microtubules

Specificity

Detects mammalian and chicken neuron-specific beta -III tubulin but not other beta -tubulin isotypes in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Neuron-specific beta-III Tubulin Antibody (TuJ-1) [NL557]

beta ‑III Tubulin antibody in Rat Cortical Stem Cells by Immunocytochemistry (ICC).

beta ‑III Tubulin in Rat Cortical Stem Cells.

beta ‑III Tubulin was detected in immersion fixed 7 day differentiated rat cortical stem cells using a 1:10 dilution of Neuron-specific beta ‑III Tubulin (Clone TuJ‑1) NorthernLights™-557 Conjugated Monoclonal Antibody (Catalog # NL1195R) for 3 hours at room temperature. Cells were stained (red) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Mouse beta-III Tubulin by Immunocytochemistry/Immunofluorescence

Detection of Mouse beta-III Tubulin by Immunocytochemistry/Immunofluorescence

Assessment of the roles of macrophages in corneal development. a Co-staining of limbal blood vessels/corneal nerve fibers and macrophages in mice at postnatal day 7 (P7). The upper image shows the immunostaining of the limbal blood vessels and macrophages with anti-CD31 antibody and anti-CD64 antibody (scale bar, 10 μm). The lower image shows the immunostaining of the corneal nerve fibers and macrophages with anti-beta -III-tubulin antibody and anti-CD64 antibody (scale bar, 10 μm). b The gating strategies of analyzing corneal macrophages with flow cytometry. Corneal macrophages in mice at P7 were classified into C–C chemokine receptor type 2 negative (CCR2−) and positive (CCR2+) populations. The gating strategies were justified by the related isotype controls in Supplementary Fig. 6. c The expression of genes involved in cell division (left image) and neurogenesis and angiogenesis (right image) in the flow-cytometry-sorted corneal CCR2− and CCR2+ macrophages with qPCR (n = 3 independent experiments, ten mice per experiment in each group). d Changes in the corneal development of anti-CSF1R antibody-treated mice (n = 6 mice at each time point in each group). e Alteration of the expression of genes involved in cell division, neurogenesis, and angiogenesis in corneal tissues of anti-CSF1R antibody-treated mice at P28 (n = 4 independent experiments, six mice per experiment in each group). f Changes in the corneal development of BMS CCR2 22-treated mice (n = 6 mice at each time point in each group). The data are presented as mean ± SD. *P < 0.05, **P < 0.01 and ***P < 0.001 Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31434991), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Neuron-specific beta-III Tubulin Antibody (TuJ-1) [NL557]

Application
Recommended Usage

Immunocytochemistry

1:10 dilution
Sample: Immersion-fixed 7 day differentiated rat cortical stem cells

Reviewed Applications

Read 1 review rated 5 using NL1195R in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied as a 10X solution of antibody in PBS containing sodium azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: beta-III Tubulin

beta -III Tubulin, also known as tubulin beta -4, is regarded as a neuron-specific marker. The expression of beta -III Tubulin has been suggested to be one of the earliest markers to signal commitment in primitive neuroepithelium.

Alternate Names

beta-Tubulin III, Beta3-tubulin, betaIII Tubulin, Class III beta-tubulin, TUBB3, Tubulin Beta 3, Tubulin beta-4, Tubulin beta-III

Entrez Gene IDs

10381 (Human)

Gene Symbol

TUBB3

Additional beta-III Tubulin Products

Product Documents for Neuron-specific beta-III Tubulin Antibody (TuJ-1) [NL557]

Certificate of Analysis

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Product Specific Notices for Neuron-specific beta-III Tubulin Antibody (TuJ-1) [NL557]

For research use only

Citations for Neuron-specific beta-III Tubulin Antibody (TuJ-1) [NL557]

Customer Reviews for Neuron-specific beta-III Tubulin Antibody (TuJ-1) [NL557] (1)

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  • Neuron-specific beta -III Tubulin NorthernLights™ NL557-conjugated Antibody
    Name: Nan Gao
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: corneas
    Species: Mouse
    Verified Customer | Posted 10/27/2017
    Neuron-specific beta-III Tubulin Antibody (TuJ-1) [NL557] NL1195R

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