NFATC1/NFAT2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-57739

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all NFATC1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: GYGAALDGGPAGYFLSSGHTRPDGAPALESPRIEITSCLGLYHNNNQFFHDVEVEDVLPSSKRSPSTATLSLPSLEAYRD

Reactivity Notes

Rat 84%. Use in Mouse reported in secitific publication PMID: 32729236

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NFATC1/NFAT2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: NFATC1/NFAT2 Antibody [NBP2-57739]

Immunocytochemistry/ Immunofluorescence: NFATC1/NFAT2 Antibody [NBP2-57739]

Immunocytochemistry/Immunofluorescence: NFATC1/NFAT2 Antibody [NBP2-57739] - Staining of human cell line MCF7 shows localization to nucleoplasm & nuclear bodies.
NFATC1/NFAT2 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: NFATC1/NFAT2 Antibody - BSA Free [NBP2-57739]

Chromatin Immunoprecipitation-exo-Seq: NFATC1/NFAT2 Antibody - BSA Free [NBP2-57739]

ChIP-Exo-Seq composite graph for Anti-NFATC1 (NBP2-57739) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.
NFATC1/NFAT2 Antibody - BSA Free

Western Blot: NFATC1/NFAT2 Antibody - BSA Free [NBP2-57739] -

Effects of low‐dose lithium supplementation on soleus and extensor digitorum longus (EDL) NFAT activation, PGC‐1 alpha and MHC isoform expression. (a) Representative Western blot images of phosphorylated and total nuclear factor activated T cells (p‐NFATc1 and NFATc1) and peroxisome proliferator‐activated receptor gamma coactivator 1‐alpha (PGC1‐ alpha ), all of which are downstream markers of calcineurin signaling. For pNFATc1, NFATc1, and PGC‐1 alpha, 7.5 μg of total protein was loaded into each well. (b) Quantification of p‐NFATc1 and NFATC1 were first normalized to ponceau prior to calculating the p‐NFATc1/NFATc1 ratio. (c) Quantification of PGC1‐ alpha content in soleus and EDL muscles in control‐fed and LiCl groups normalized to ponceau. (d) Representative Western blot images of MHC I and IIa in soleus muscles from control and lithium‐fed mice. For both isoforms, 5 μg of total protein was loaded into each well. Quantification of MHC I (e) and MHC IIa (f) normalized to ponceau. For (b,c) and (e,f), all data are expressed relative to control. *p <.05, significantly different from control, using a Student's t tests (n = 6–9 per group). All data are expressed as means +/- SD Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32729236), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for NFATC1/NFAT2 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml
Application Notes
ICC/IF, Fixation/Permeabilization: PFA/Triton X-100

Reviewed Applications

Read 1 review rated 3 using NBP2-57739 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NFATC1

The nuclear factor of activated T-cells (NFAT) transcription complex is required for the expression of a group of proteins that coordinate the immune response. NFAT2 is the cytosolic component of NFAT and is encoded by a separate gene. Expression of NFAT2 activates the interleukin (IL-2) promoter in non-T lymphocytes and is required for IL-2 gene expression.

Long Name

Nuclear Factor of Activated T Cells C1

Alternate Names

NFAT2

Gene Symbol

NFATC1

Additional NFATC1 Products

Product Documents for NFATC1/NFAT2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for NFATC1/NFAT2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for NFATC1/NFAT2 Antibody - BSA Free

Customer Reviews for NFATC1/NFAT2 Antibody - BSA Free (1)

3 out of 5
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  • NFATC1/NFAT2 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Mouse skeletal muscle
    Species: Mouse
    Verified Customer | Posted 06/15/2017
    1:2500 primary dilution overnight in milk 1:2000 secondary dilution 1 hour in milk
    NFATC1/NFAT2 Antibody - BSA Free NBP2-57739

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