NFYB Antibody (6H6) - Azide and BSA Free

Novus Biologicals | Catalog # H00004801-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 6H6

Format

Azide and BSA Free
View all NFYB Primary Antibodies »

Product Specifications

Immunogen

NFYB (AAH05317, 1 a.a. ~ 207 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MTMDGDSSTTDASQLGISADYIGGSHYVIQPHDDTEDSMNDHEDTNGSKESFREQDIYLPIANVARIMKNAIPQTGKIAKDAKECVQECVSEFISFITSEASERCHQEKRKTINGEDILFAMSTLGFDSYVEPLKLYLQKFREAMKGEKGIGGAVTATDGLSEELTEEAFTNQLPAGLITTDGQQQNVMVYTTSYQQISGVQQIQFS

Specificity

NFYB - nuclear transcription factor Y, beta

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for NFYB Antibody (6H6) - Azide and BSA Free

Western Blot: NFYB Antibody (6H6) [H00004801-M01]

Western Blot: NFYB Antibody (6H6) [H00004801-M01]

Western Blot: NFYB Antibody (6H6) [H00004801-M01] - Analysis of NFYB expression in transfected 293T cell line by NFYB monoclonal antibody (M01), clone 6H6.Lane 1: NFYB transfected lysate(22.8 KDa).Lane 2: Non-transfected lysate.
Western Blot: NFYB Antibody (6H6) [H00004801-M01]

Western Blot: NFYB Antibody (6H6) [H00004801-M01]

Western Blot: NFYB Antibody (6H6) [H00004801-M01] - Analysis of NFYB over-expressed 293 cell line, cotransfected with NFYB Validated Chimera RNAi ( Cat # H00004801-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NFYB monoclonal antibody (M01), clone 6H6 (Cat # H00004801-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Sandwich ELISA: NFYB Antibody (6H6) [H00004801-M01] - Detection limit for recombinant GST tagged NFYB is approximately 0.1ng/ml as a capture antibody.

Applications for NFYB Antibody (6H6) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NFYB

The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants.

Alternate Names

CAAT box DNA-binding protein subunit B, CBF-A, HAP3CBF-B, NF-YBCCAAT-binding transcription factor subunit A, Nuclear transcription factor Y subunit B, nuclear transcription factor Y subunit beta, nuclear transcription factor Y, beta, Transcription factor NF-Y, B subunit

Entrez Gene IDs

4801 (Human)

Gene Symbol

NFYB

OMIM

189904 (Human)

UniProt

AAH05317 (Human)

Additional NFYB Products

Product Documents for NFYB Antibody (6H6) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for NFYB Antibody (6H6) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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