![Immunohistochemistry-Paraffin: NFYB Antibody [NBP2-19535]](https://resources.rndsystems.com/images/products/NFYB-Antibody-Immunohistochemistry-Paraffin-NBP2-19535-img0005.jpg "Immunohistochemistry-Paraffin: NFYB Antibody [NBP2-19535]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
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Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Zebrafish
Predicted:
Bovine (98%), Porcine (99%), Rhesus Macaque (100%), Xenopus (92%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry Whole-Mount, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human NFYB. The exact sequence is proprietary.
Reactivity Notes
Xenopus laevis (92%).
Localization
Nucleus
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for NFYB Antibody
Immunohistochemistry-Paraffin: NFYB Antibody [NBP2-19535]
Immunohistochemistry-Paraffin: NFYB Antibody [NBP2-19535] - Rat stomach. NFYB antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.![Immunohistochemistry-Paraffin: NFYB Antibody [NBP2-19535]](https://resources.rndsystems.com/images/products/NFYB-Antibody-Immunohistochemistry-Paraffin-NBP2-19535-img0004.jpg "Immunohistochemistry-Paraffin: NFYB Antibody [NBP2-19535]")
Immunohistochemistry-Paraffin: NFYB Antibody [NBP2-19535]
Immunohistochemistry-Paraffin: NFYB Antibody [NBP2-19535] - Mouse intestine. NFYB antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: NFYB Antibody [NBP2-19535] -
Western Blot: NFYB Antibody [NBP2-19535] - Various tissue extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with NFYB antibody (NBP2-19535) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Immunohistochemistry: NFYB Antibody [NBP2-19535] -
Immunohistochemistry: NFYB Antibody [NBP2-19535] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using NFYB antibody (NBP2-19535) at 1:300 dilution.
Western Blot: NFYB Antibody [NBP2-19535] -
Western Blot: NFYB Antibody [NBP2-19535] - HeLa whole cell and nuclear extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with NFYB antibody (NBP2-19535) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: NFYB Antibody [NBP2-19535] -
Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with NFYB antibody (NBP2-19535) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: NFYB Antibody [NBP2-19535] -
NFYB antibody detects NFYB protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NFYB protein stained by NFYB antibody (NBP2-19535) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar = 10 um.
Western Blot: NFYB Antibody [NBP2-19535] -
Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with NFYB antibody (NBP2-19535) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry: NFYB Antibody [NBP2-19535] -
Immunohistochemical analysis (whole mount) of zebrafish embryo, using NFYB antibody (NBP2-19535) at 1:200 dilution.
Chromatin Immunoprecipitation: NFYB Antibody [NBP2-19535] -
Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control rabbit IgG or anti-NFYB antibody. The precipitated DNA was detected by PCR with primer set targeting to aurora kinase A.
Immunoprecipitation: NFYB Antibody [NBP2-19535] -
NFYB antibody immunoprecipitates NFYB protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 40 ug whole cell lysate/extract of NFYB protein expressing 293T cells B : Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of NFYB protein by 2.5 ug of NFYB antibody (NBP2-19535) 10% SDS-PAGE The immunoprecipitated NFYB protein was detected by NFYB antibody (NBP2-19535) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.Applications for NFYB Antibody
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
Assay dependent
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry Whole-Mount
Assay dependent
Immunohistochemistry-Paraffin
1:100-1:1000
Immunoprecipitation
1:100-1:500
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1% BSA, 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: NFYB
Alternate Names
CAAT box DNA-binding protein subunit B, CBF-A, HAP3CBF-B, NF-YBCCAAT-binding transcription factor subunit A, Nuclear transcription factor Y subunit B, nuclear transcription factor Y subunit beta, nuclear transcription factor Y, beta, Transcription factor NF-Y, B subunit
Gene Symbol
NFYB
UniProt
Additional NFYB Products
Product Documents for NFYB Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for NFYB Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ChIP Protocol Video
- Chromatin Immunoprecipitation (ChIP) Protocol
- Chromatin Immunoprecipitation Protocol
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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