Nitrotyrosine Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-16015

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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all Nitrotyrosine Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence containing myristylated K.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Nitrotyrosine Antibody - Azide and BSA Free

Western Blot: Nitrotyrosine AntibodyAzide and BSA Free [NBP3-16015]

Western Blot: Nitrotyrosine AntibodyAzide and BSA Free [NBP3-16015]

Western Blot: Nitrotyrosine Antibody [NBP3-16015] - Western blot analysis of extracts of various cell lines, using Nitrotyrosine antibody (NBP3-16015) at 1:1000 dilution.HeLa and C6 cells were treated by Perxynitrate(3mM)for 1 hours.Jurkat cells were treated by Pervanadate (1 mM) at 37C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Western Blot: Nitrotyrosine AntibodyAzide and BSA Free [NBP3-16015]

Western Blot: Nitrotyrosine AntibodyAzide and BSA Free [NBP3-16015]

Western Blot: Nitrotyrosine Antibody [NBP3-16015] - Western blot analysis of extracts of NIH/3T3 cells, using Nitrotyrosine antibody (NBP3-16015) at 1:1000 dilution.NIH3T3 cells were treated by Perxynitrate(3mM)for 3 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Applications for Nitrotyrosine Antibody - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Nitrotyrosine

Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling (2). While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophilperoxidase (3), myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Nitrotyrosine may also serve as a biomarker for the effects of reactive nitrogen oxides, based on tyrosine residues becoming nitrated in proteins at sites of inflammation induced tissue injury (1). The presence of nitro tyrosine-containing proteins therefore has shown high correlation to disease states such as atherosclerosis, Alzheimer's disease, Parkinson's disease and amyotrophic lateral sclerosis (4).

Alternate Names

Nitrotyrosine

Additional Nitrotyrosine Products

Product Documents for Nitrotyrosine Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Nitrotyrosine Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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