NMDAR2A Antibody (S327A-38) - BSA Free
Novus Biologicals | Catalog # NBP2-22404
![Western Blot: NMDAR2A Antibody (S327A-38) [NBP2-22404]](https://resources.rndsystems.com/images/products/NMDAR2A-Antibody-S327A-38-Western-Blot-NBP2-22404-img0002.jpg "Western Blot: NMDAR2A Antibody (S327A-38) [NBP2-22404]")
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # S327A-38
Format
BSA Free
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Product Specifications
Immunogen
Fusion protein amino acids 75-325 (extracellular N-terminus) of rat GluN2A/NR2A
Localization
Cell Membrane, Cell Junction
Specificity
Detects approx 170kDa. Does not react with NR2B.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for NMDAR2A Antibody (S327A-38) - BSA Free
Western Blot: NMDAR2A Antibody (S327A-38) [NBP2-22404]
Western Blot: NMDAR2A Antibody (S327A-38) [NBP2-22404] - Western Blot analysis of Monkey COS cells transfected with GFP-tagged NMDAR2A showing detection of ~170 kDa NMDAR2A protein using Mouse Anti-GluN2A/NMDAR2A Monoclonal Antibody, Clone S327A-38 (NBP2-22404). Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with GFP-tagged NMDAR2A. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-NMDAR2A Monoclonal Antibody (NBP2-22404) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~170 kDa.![Immunocytochemistry/ Immunofluorescence: NMDAR2A Antibody (S327A-38) [NBP2-22404]](https://resources.rndsystems.com/images/products/NMDAR2A-Antibody-S327A-38-Immunocytochemistry-Immunofluorescence-NBP2-22404-img0003.jpg "Immunocytochemistry/ Immunofluorescence: NMDAR2A Antibody (S327A-38) [NBP2-22404]")
Immunocytochemistry/ Immunofluorescence: NMDAR2A Antibody (S327A-38) [NBP2-22404]
Immunocytochemistry/Immunofluorescence: NMDAR2A Antibody (S327A-38) [NBP2-22404] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GluN2A/NMDAR2A Monoclonal Antibody, Clone S327A-38 (NBP2-22404). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GluN2A/NMDAR2A Monoclonal Antibody (NBP2-22404) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GluN2A/NMDAR2A Antibody (D) Composite.![Immunocytochemistry/ Immunofluorescence: NMDAR2A Antibody (S327A-38) [NBP2-22404]](https://resources.rndsystems.com/images/products/NMDAR2A-Antibody-S327A-38-Immunocytochemistry-Immunofluorescence-NBP2-22404-img0004.jpg "Immunocytochemistry/ Immunofluorescence: NMDAR2A Antibody (S327A-38) [NBP2-22404]")
Immunocytochemistry/ Immunofluorescence: NMDAR2A Antibody (S327A-38) [NBP2-22404]
Immunocytochemistry/Immunofluorescence: NMDAR2A Antibody (S327A-38) [NBP2-22404] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GluN2A/NMDAR2A Monoclonal Antibody, Clone S327A-38 (NBP2-22404). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GluN2A/NMDAR2A Monoclonal Antibody (NBP2-22404) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Cytoplasm. Magnification: 60X. (A) Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain. (B) Anti-GluN2A/NMDAR2A Antibody. (C) Composite. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) GluN2A/NMDAR2A Antibody. (D) Composite.Applications for NMDAR2A Antibody (S327A-38) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:1000
Application Notes
1 ug/ml of GluN2A/NR2A Antibody was sufficient for detection of GluN2A/NR2A in 20 ug of COS cells transiently transfected with GFP-tagged NR2A lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.4), 50% Glycerol
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: NMDAR2A
Long Name
Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate, Subunit 2A
Alternate Names
EPND, FESD, GluN2A, glutamate receptor, ionotropic, N-methyl D-aspartate 2A, hNR2A, LKS, NMDA R, NR2A Subunit, NMDA receptor subtype 2A, NMDAR2A, N-methyl D-aspartate receptor subtype 2A, N-methyl-D-aspartate receptor subunit 2A, NR2A, subunit epsilon-1
Gene Symbol
GRIN2A
UniProt
Additional NMDAR2A Products
Product Documents for NMDAR2A Antibody (S327A-38) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for NMDAR2A Antibody (S327A-38) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for NMDAR2A Antibody (S327A-38) - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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