NOX1 Antibody - Azide Free

Novus Biologicals | Catalog # NBP1-31546

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation, Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, IHC-P WB

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide Free
View all NOX1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human NOX1. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (85%).

Localization

Membrane, Multi-pass membrane protein

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NOX1 Antibody - Azide Free

Western Blot: NOX1 Antibody [NBP1-31546]

Western Blot: NOX1 Antibody [NBP1-31546]

NOX1-Antibody-Western-Blot-NBP1-31546-img0008.jpg
Immunohistochemistry-Paraffin: NOX1 Antibody [NBP1-31546]

Immunohistochemistry-Paraffin: NOX1 Antibody [NBP1-31546]

Immunohistochemistry-Paraffin: NOX1 Antibody [NBP1-31546] - Mouse kidney. NOX1 stained by NOX1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Flow Cytometry: NOX1 Antibody [NBP1-31546]

Flow Cytometry: NOX1 Antibody [NBP1-31546]

Flow Cytometry: NOX1 Antibody [NBP1-31546] - Nox-1 is present at the surface of resting platelets. Human platelets (4 x 10^8 cells/mL) were isolated from whole blood and incubated with the Nox-1 antibody or Rabbit IgG control at 1:250 dilution for 30 minutes at room temperature. FITC-tagged anti-rabbit (secondary antibody) was added at 1:100 dilution and incubated for an additional 30 minutes. Platelets were then diluted 1:25 v/v in Tyrodes buffer and read using a BD Accuri flow cytometer. Image from verified customer review.
Western Blot: NOX1 Antibody [NBP1-31546]

Western Blot: NOX1 Antibody [NBP1-31546]

Western Blot: NOX1 Antibody [NBP1-31546] - Sample (30 ug of whole cell lysate) A: JC B: BCL-1 7. 5% SDS PAGE; antibody diluted at 1:5000.
Western Blot: NOX1 Antibody [NBP1-31546]

Western Blot: NOX1 Antibody [NBP1-31546]

Western Blot: NOX1 Antibody [NBP1-31546] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa 7. 5% SDS PAGE, antibody diluted at 1:2000.
Western Blot: NOX1 Antibody [NBP1-31546]

Western Blot: NOX1 Antibody [NBP1-31546]

Western Blot: NOX1 Antibody [NBP1-31546] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NOX1 antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: NOX1 Antibody [NBP1-31546]

Immunohistochemistry-Paraffin: NOX1 Antibody [NBP1-31546]

Immunohistochemistry-Paraffin: NOX1 Antibody [NBP1-31546] - NOX1 antibody detects NOX1 protein at cytoplasm on human lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung carcinoma.NOX1 antibody diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
NOX1 Antibody

Western Blot: NOX1 Antibody [NBP1-31546] -

Western Blot: NOX1 Antibody [NBP1-31546] - Blueberry polyphenol extract (BPE) did not affect the expression of NADPH oxidases (NOX) in angiotensin (Ang) II-treated human aortic endothelial cells (HAECs). HAECs were treated with 200 µg/mL of BPE for 1 h then treated with 200 nM of Ang II for 12 h. Protein expression of NOX1 (A,B), NOX2 (C,D), NOX4 (E,F), & NOX5 (G,H) were determined by Western blot. Quantification was performed using Image Lab (Bio-Rad Laboratories, Inc.). Data are expressed as mean ± SD from three (NOX1 & NOX4) & five (NOX2 & NOX5) independent experiments. Values that do not share the same letter are significantly different from each other (p ≤ 0.05). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35453301), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
NOX1 Antibody - Azide Free

Western Blot: NOX1 Antibody [NBP1-31546] -

Untreated (-) and treated (+) HCT116 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NOX1 antibody (NBP1-31546) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
NOX1 Antibody - Azide Free

Immunocytochemistry/ Immunofluorescence: NOX1 Antibody [NBP1-31546] -

NOX1 antibody detects NOX1 protein at cytoplasm by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NOX1 protein stained by NOX1 antibody (NBP1-31546) diluted at 1:500.
Blue: Hoechst 33342 staining.

Applications for NOX1 Antibody - Azide Free

Application
Recommended Usage

Flow Cytometry

Validated from a verified customer review.

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

Reported in scientific literature (PMID: 27094494)

Western Blot

1:500-1:3000

Reviewed Applications

Read 1 review rated 5 using NBP1-31546 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Format

Azide Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NOX1

The NOX family NADPH protein oxidases are involved in transfer of electrons accross membranes to generate reactive oxygen species. There are 7 family members - NOX1-5, DUOX1 and DUOX2. Expression of NOX1 is highest in colon tissue; it is also found in vascular tissue and can also been induced by a variety of factors including PDGF. It requires interaction with NOXO1 and NOXA1 for superoxide generation.

Long Name

NADPH Oxidase 1

Alternate Names

MOX-1, NOH1

Entrez Gene IDs

27305 (Human)

Gene Symbol

NOX1

UniProt

Q9Y5S8 (Human)

Additional NOX1 Products

Product Documents for NOX1 Antibody - Azide Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for NOX1 Antibody - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Redox Enzymes

Citations for NOX1 Antibody - Azide Free

Customer Reviews for NOX1 Antibody - Azide Free (1)

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Customer Images

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  • NOX1 Antibody
    Name: Renato Gaspar
    Application: Flow Cytometry
    Sample Tested: Platelets
    Species: Human
    Verified Customer | Posted 01/24/2019
    Nox-1 is present at the surface of resting platelets.
    Platelets (4 x 10^8 cells/mL) were isolated from whole blood and incubated with the Nox-1 antibody or Rabbit IgG control at 1:250 dilution for 30 minutes at room temperature. FITC-tagged anti-rabbit (secondary antibody) was added at 1:100 dilution and incubated for an additional 30 minutes. Platelets were then diluted 1:25 v/v in Tyrodes buffer and read using a BD Accuri flow cytometer.
    NOX1 Antibody - Azide Free NBP1-31546

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