Nrf1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-77822

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Nrf1 Primary Antibodies »

Product Specifications

Immunogen

This protein A purified Nrf1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a purified recombinant mouse Nrf1 protein corresponding to aa 1- 534 of the native protein. (Uniprot: Q9WU00)

Reactivity Notes

BLAST analysis was used to suggest reactivity with this protein from mouse, human, chimpanzee, dog, rat, chicken, frog and zebrafish based on very high sequence homology with the immunogen sequence. Cross reactivity with Nrf1 homologues from other sources has not been determined.

Specificity

This protein A purified antibody is directed against mouse Nrf1.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This protein A purified antibody is directed against mouse Nrf1. The product was purified from monospecific antiserum by protein A affinity purification

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for Nrf1 Antibody - BSA Free

Western Blot: Nrf1 Antibody [NBP1-77822]

Western Blot: Nrf1 Antibody [NBP1-77822]

Western Blot: Nrf1 Antibody [NBP1-77822] - Lane: NFR1-HIS recombinant protein. Load: 50ug per lane. Primary antibody - 1: NRF1 antibody at 1:1,000 overnight at 4C. Primary antibody - 2: 6xHIS Epitope tag antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/observed size: 67 kDa, 67 kDa for NRF1-His tagged. Other band(s): None.
Immunohistochemistry: Nrf1 Antibody [NBP1-77822]

Immunohistochemistry: Nrf1 Antibody [NBP1-77822]

Immunohistochemistry: Nrf1 Antibody [NBP1-77822] - Used at a 5 ug/ml to detect NRF1 in a variety of tissues. This image shows NRF1 staining of human testis. The antibody shows nuclear staining in lymphocytes. Tissue was formalin-fixed and paraffin embedded.
Western Blot: Nrf1 Antibody [NBP1-77822]

Western Blot: Nrf1 Antibody [NBP1-77822]

Western Blot: Nrf1 Antibody [NBP1-77822] - Shows detection of a 67-kDa band corresponding to human NRF1 in a (lane 1) HeLa nuclear extract and (lane 2) whole cell lysate (molecular weight marker not shown). Approx. 10 ug of each lysate was separated by SDS-PAGE and transferred onto nitrocellulose. The blot was incubated with a 1:500 dilution of the antibody at room temperature for 1 h followed by detection using IRDye (TM) 700 labeled Goat-a-Rabbit IgG [H&L] diluted 1:2,500. IRDye (TM) 700
Nrf1 Antibody

Nrf1 Antibody

Affinity Purified anti-NRF1antibody was used at a 5 ug/ml to detect NRF1 in a variety of tissues. This image shows NRF1 staining of human testis. The antibody shows nuclear staining in lymphocytes. Tissue was formalin-fixed and paraffin embedded. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.
Nrf1 Antibody - BSA Free

Western Blot: Nrf1 Antibody - BSA Free [NBP1-77822] -

Immunoblotting analysis of mitochondrial biogenesis. (A) Representative immunoblots for mitochondrial biogenesis proteins in hAbKI mice and treated hAbKI mice with Urolithin A and EGCG. (B) Quantitative-densitometry analysis of mitochondrial biogenesis proteins PGC1 alpha, NRF1, NRF2 and TFAM. PGC1 alpha, NRF1, NRF2 and TFAM were significantly increased in the treated hAbKI mice Urolithin A and EGCG. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36078067), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Nrf1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:3000-1:10000

Immunohistochemistry

2 mg/ml-5 ug/ml

Immunohistochemistry-Paraffin

2 mg/ml-5 ug/ml

Western Blot

1:500-1:2000
Application Notes
This protein A purified antibody has been tested for use in ELISA, immunohistochemistry and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 67 kDa in size corresponding to NRF1 by western blotting in the appropriate cell lysate or extract. Splice variants exist for this protein that may result in the detection of lower molecular weight bands.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: Nrf1

NRF1 (also known as nuclear respiratory factor 1, alpha palindromic binding protein and alpha-pal) is the mammalian homolog to the erect wing (ewg) Drosophila protein that is required for proper development of the central nervous system and indirect flight muscles. In mammals NRF1 functions as a transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. This protein links the transcriptional modulation of key metabolic genes to cellular growth and development and has been implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. NRF1 forms a homodimer and binds DNA as a dimer. NRF1 shows a nuclear localization and is widely expressed in embryonic, fetal, and adult tissues. Phosphorylation of NRF1 enhances DNA binding. Multiple splice variants have been identified for this protein.

Long Name

NF-E2-related Factor 1

Alternate Names

HBZ17, LCR-F1, NFE2L1, TCF11

Gene Symbol

NRF1

UniProt

Q9WU00 (Mouse)

Additional Nrf1 Products

Product Documents for Nrf1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Nrf1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Nrf1 Antibody - BSA Free

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Protocols

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