Nuf2 Antibody (24GB570)

Novus Biologicals | Catalog # NBP3-50539

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 24GB570
View all Nuf2 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human Nuf2 (Uniprot: Q9BZD4)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Nuf2 Antibody (24GB570)

Nuf2 Antibody (24GB570)

Flow Cytometry: Nuf2 Antibody (24GB570)[NBP3-50539] -

Flow cytometric analysis of Nuf2expression in HepG2 cells using Nuf2 antibody (1:2,000). Green, isotype control; red, NUF2.
Nuf2 Antibody (24GB570)

Western Blot: Nuf2 Antibody (24GB570)[NBP3-50539] -

Western blotting analysis using Nuf2 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for Nuf2 Antibody (24GB570)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Nuf2

Nuf2, also known as Kinetochore protein Nuf2, is a 54 kDa 464 amino acid protein, and it is involved in chromosome segregation, specifically as it relates to spindle checkpoint activity with the kinetochore-associated NDC80 complex. This protein is necessary for kinetochore stability and organization of microtubule binding sites on the outer plate, forming interactions with NDC80/HEC-1-CDCA1, SPBC24-SPBC25, Bub1, EXOC1, as well as AURKB/Aurora-B and CENPE in the mitotic prometaphase, DNA replication, and Cell Cycle pathways. Current research is being performed on this proteins involvement in gastric, lung, and breast cancers, in addition to essential hypertension, schizophrenia, and duodenogastric reflux.

Alternate Names

cancer/testis antigen 106, CDCA1hsNuf2, cell division cycle associated 1, Cell division cycle-associated protein 1, CT106, hNuf2, kinetochore protein Nuf2, NUF2, NDC80 kinetochore complex component, homolog (S. cerevisiae), NUF2RhNuf2R

Gene Symbol

NUF2

Additional Nuf2 Products

Product Documents for Nuf2 Antibody (24GB570)

Certificate of Analysis

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Product Specific Notices for Nuf2 Antibody (24GB570)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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