OATP2/8 Antibody (MDQ)

Novus Biologicals | Catalog # NB100-74482

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # MDQ
View all OATP2/8 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to residues M(1) D Q N Q H L N K T A E A Q P S E N K K T R Y(23) C of human OATP2.

Epitope

The exact epitope within the immunizing peptide sequence is unknown.

Specificity

NB100-74482 detects OATP2 and OATP8 from human tissue and cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

This product was originally labeled as an antibody to OATP8. However, the amino acid sequence used as the immunizing peptide is 100% identical to OATP2. Furthermore, publications using this antibody show reactivity to both OATP2 and 8. As such, this antibody is reactive to both OATP2 and OATP8.

Scientific Data Images for OATP2/8 Antibody (MDQ)

Immunocytochemistry/ Immunofluorescence: OATP2/8 Antibody (MDQ) [NB100-74482]

Immunocytochemistry/ Immunofluorescence: OATP2/8 Antibody (MDQ) [NB100-74482]

Immunocytochemistry/Immunofluorescence: OATP2/8 Antibody (MDQ) [NB100-74482] - Analysis of OATP8 in HepG2 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a OATP8 monoclonal antibody at a dilution of 1:20 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. OATP8 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.
Immunohistochemistry: OATP2/8 Antibody (MDQ) [NB100-74482]

Immunohistochemistry: OATP2/8 Antibody (MDQ) [NB100-74482]

OATP2-8-Antibody-MDQ-Immunohistochemistry-NB100-74482-img0003.jpg
OATP2/8 Antibody (MDQ)

Immunohistochemistry: OATP2/8 Antibody (MDQ) [NB100-74482] -

a–l Immunohistochemical staining of liver transporters in the resected specimens from our case and control cases

Applications for OATP2/8 Antibody (MDQ)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Frozen

1:20

Immunohistochemistry-Paraffin

1:20

Immunoprecipitation

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
WB usage w as reported in literature (See Cui Y et al.). IHC-P usage reported in scientific literature (PMID: 25063395).

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Tissue culture supernatant

Preservative

0.09% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: OATP2/8

The OATPs (organic anion transporter polypeptides) are a family of transport proteins that have been found to mediate the uptake of organic ions into hepatocyte cells. These proteins fall into the solute carrier family 21A (SLC21A), and are expressed in liver, kidney, and brain. These polypeptides are involved in the excretion of potentially toxic compounds and therefore play a role in the overall detoxification system of the body. Unlike other members of the OATP family, OATP2 and 8 have been detected exclusively in the liver, and there is an 80% amino acid sequence homology between these polypeptides. Other names for OATP2 include SLC21A6, OATP-C, and LST-1.

Alternate Names

HBLRR, LST1, LST-1, LST-2, LST3, LST-3TM13, OATP1B1, OATP1B3, OATP2, OATP8, OATP-8, OATPC, OATP-C, SLC21A6, SLC21A8, SLCO1B1 solute carrier organic anion transporter family, member 1B1

Entrez Gene IDs

10599 (Human); 28234 (Human)

Gene Symbol

SLCO1B1

UniProt

Q9Y6L6 (Human)

Additional OATP2/8 Products

Product Documents for OATP2/8 Antibody (MDQ)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for OATP2/8 Antibody (MDQ)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for OATP2/8 Antibody (MDQ)

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for OATP2/8 Antibody (MDQ)

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  • Q: The customer is very confused with product name of #NB100-74482. SLC01B3(MDQ/5F260) was indicated on the vial label. SLC01B3 is alternative name of OATP8. But OATP2(MDQ) was indicated on the datasheet on your web. Furthermore, when the customer searched 5F260 on your web, #NB600-1158 (OATP8 Antibody (5F260)) appeared. I think something is wrong for #NB100-74482. Could you confirm the product name of suppliers' and let us know? As far as I looked the Internet, Clone MDQ reacts with both OATP2 and OATP8. Clone 5F260 also reacts with both OATP2 and OATP8? Is MDQ identical with 5F260?

    A: Clone MDQ and clone 5F260 are two separate clones generated using the same immunogen. Both are also predicted to react with both OATP2 and OATP8, based on the sequence similarity.

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