OPA1 Antibody (1284B) - BSA Free

Novus Biologicals | Catalog # NBP2-59770

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1284B expressed in HEK293

Format

BSA Free
View all OPA1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an internal region within residues 500-600 of human OPA1. [Swiss-Prot# O60313]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for OPA1 Antibody (1284B) - BSA Free

Western Blot: OPA1 Antibody (1284B)BSA Free [NBP2-59770]

Western Blot: OPA1 Antibody (1284B)BSA Free [NBP2-59770]

Western Blot: OPA1 Antibody (1284B) [NBP2-59770] - Total protein from Mouse Neuro2A cells, Human HeLa cells and Rat PC12 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-OPA1 in blocking buffer, and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Immunocytochemistry/ Immunofluorescence: OPA1 Antibody (1284B) - BSA Free [NBP2-59770]

Immunocytochemistry/ Immunofluorescence: OPA1 Antibody (1284B) - BSA Free [NBP2-59770]

Immunocytochemistry/Immunofluorescence: OPA1 Antibody (1284B) [NBP2-59770] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with OPA1 Antibody [1284B] conjugated to Biotin (NBP2-59770B) at 5 ug/ml for 1 hour at room temperature then detected with Streptavidin conjugated to DyLight 550. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: OPA1 Antibody (1284B) - BSA Free [NBP2-59770]

Immunohistochemistry-Paraffin: OPA1 Antibody (1284B) - BSA Free [NBP2-59770]

Immunohistochemistry-Paraffin: OPA1 Antibody (1284B) [NBP2-59770] - IHC analysis of a formalin fixed paraffin embedded (FFPE) tissue section of mouse testes using OPA1 antibody at 1:1000 dilution. The primary antibody bound to OPA1 antigens in the tissue section was deteced using an HRP labeled secondary antibody and DAB reagent. Nuclei of the cells were counterstained with hematoxylin. This OPA1 antibody generated a diffused cytoplasmic staining of OPA1 protein in the tubular epithelial cells, the spermatids/spermatocytes and Leydig's cells.
Immunocytochemistry/ Immunofluorescence: OPA1 Antibody (1284B) - BSA Free [NBP2-59770]

Immunocytochemistry/ Immunofluorescence: OPA1 Antibody (1284B) - BSA Free [NBP2-59770]

Immunocytochemistry/Immunofluorescence: OPA1 Antibody (1284B) [NBP2-59770] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-OPA1 at 5 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Applications for OPA1 Antibody (1284B) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

5 ug/ml

Immunohistochemistry

1:1000

Immunohistochemistry-Paraffin

1:1000

Western Blot

2 ug/ml

Reviewed Applications

Read 1 review rated 4 using NBP2-59770 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: OPA1

OPA1 (optic atrophy protein 1) is a dynamin-related GTPase which forms a diffusion barrier for proteins stored in mitochondrial cristae and is critical to mitochondrial fusion as well as regulation of apoptosis. Intrinsic apoptotic signals mediated proteolytic processing leads OPA1 oligomers disassembly and caspase activator cytochrome C (CYCS) release into mitochondrial intermembrane spaces. Under conditions of stress, dynamin-like 120 kD protein, form S1 (which is the inactive form produced via cleavage at S1 position by OMA1) triggers the loss of mitochondrial membrane potential that results in negative regulation of the process of mitochondrial fusion. OPA1 exists as oligomeric complex consisting of membrane-bound and soluble forms of OPA1, and the latter can interacts with CHCHD3, IMMT and PARL. PARL-dependent proteolytic processing releases an antiapoptotic soluble form which is not required for mitochondrial fusion. Defects in OPA1 are a cause of optic atrophy type 1 (OPA1) as well as dominant optic atrophy plus syndrome (DOA+)

Long Name

Optic Atrophy Protein 1

Alternate Names

BERHS, EC 3.6.5.5, LargeG, lilr3, MGM1, MTDPS14, NPG, NTG

Gene Symbol

OPA1

Additional OPA1 Products

Product Documents for OPA1 Antibody (1284B) - BSA Free

Certificate of Analysis

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Product Specific Notices for OPA1 Antibody (1284B) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for OPA1 Antibody (1284B) - BSA Free

Customer Reviews for OPA1 Antibody (1284B) - BSA Free (1)

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  • OPA1 Antibody (1284B)
    Name: Joshua Harvey
    Application: Western Blot
    Sample Tested: fibroblasts
    Species: Human
    Verified Customer | Posted 02/01/2021
    Cyclohexamide chase of WT and Mutant fibroblast lines. OPA1 bands were seen at 120 and 80kDa. BIII loading control was used.
    SDS-PAGE run on a 8% Bis-Tris gel. Run in a biorad cassette system Loading gel - 100V then 120V to completion. Transfer 90minutes at 100V Blocked for 1 hour at RT with 5% Milk in PBS-T Primary: Stained overnight at 4oC with 1 in 1,000 OPA1 ab with 5% Milk in PBS-T Secondary: Rabbit anti-Goat. 1 in 30,000. Stained at RT for 1 hr with 5% Milk in PBS-T Loading control: 1 in 10,000 B-tubulin (Goat). Imaged using BioRad
    OPA1 Antibody (1284B) - BSA Free NBP2-59770

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