Recombinant Monkey Osteopontin/OPN Protein

Novus Biologicals | Catalog # NBP3-57593

Novus Biologicals
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Key Product Details

Applications

Western Blot, ELISA, Cell Culture
View all Osteopontin/OPN Proteins and Enzymes »

Product Specifications

Description

A recombinant protein corresponding to 298 aa of Monkey Osteopontin/OPN.

Source: Yeast

Amino Acid Sequence: LPVKQADSGS SEEKQLYNKY PDAVATWLKP DPSQKQNLLA PQNAVSSEET NDFKQETLPS KSNESHDHMD DVDDEDDDDH VDSQDSIDSN DSDEVDDTDD SHQSDESHHS DESDELVTDF PTDLPATEVF TPVVPTVDIY DGRGDSVAYG LRSKSKKFRR PDIQYPDATD EDITSHVESE ELNGAYKAIP VVQGLNVPSD WDSRGKDSHE TSQLDDHSAE THSHKHSRLY KRKASDDSNE HSDVIDSQER SKISHEFHSH EFHSHEDMLV VDPKSKEEDK HLKFRISHEL DSASSEVN (298)[Met211Thr]

Stable for up to 12 months from date of receipt at -20C. Stable for 3 months when stored in working aliquots with a carrier protein at -20C. Avoid repeated freeze/thaw cycles.

Purity

>98%, by SDS-PAGE

Endotoxin Level

No affinity tags & naturally endotoxin-free

Predicted Molecular Mass

33.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Applications

Enzyme-linked Immunosorbent Spot (ELISPOT)
ELISA Standard (Matched Pair) (ELISA(Sta))

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation, and Storage

NBP3-57593
Formulation Lyophilized in 1-5X PBS containing 10% Trehalose
Preservative 10% Trehalose
Concentration Lyoph
Reconstitution Reconstitute in sterile PBS with at least 0.1% carrier protein
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Osteopontin/OPN

Osteopontin is the principal phosphorylated glycoprotein of bone and is expressed in a limited number of other tissues including dentine. Osteopontin is produced by osteoblasts under stimulation by calcitriol and binds tightly to hydroxyapatite. It is also involved in the anchoring of osteoclasts to the mineral of bone matrix via the vitronectin receptor, which has specificity for osteopontin. Osteopontin is overexpressed in a variety of cancers, including lung, breast, colorectal, stomach, ovarian, melanoma and mesothelioma.

Long Name

Secreted Phosphoprotein 1 [BNSP]

Alternate Names

Eta-1, OPN, Spp1

Gene Symbol

SPP1

Additional Osteopontin/OPN Products

Product Documents for Recombinant Monkey Osteopontin/OPN Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Monkey Osteopontin/OPN Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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