Human Otoferlin - Ready-To-Use ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP3-53811

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, tissue homogenates and other biological fluids (100 uL)

Sensitivity

0.064 ng/mL (example only; lot dependent)

Assay Range

0.156 - 10 ng/mL (example only; lot dependent)
View all Otoferlin ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Description

The Ready-To-Use ELISA kit offers pre-diluted detection reagents and a shorter experimental time.
Assay Length: 3 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)

Scientific Data Images for Human Otoferlin - Ready-To-Use ELISA Kit (Colorimetric)

Human Otoferlin - Ready-To-Use ELISA Kit (Colorimetric) ELISA: Human Otoferlin  - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-53811]

ELISA: Human Otoferlin  - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-53811]

Kit Contents for Human Otoferlin - Ready-To-Use ELISA Kit (Colorimetric)

  • Pre-coated 96T strip plate, Plate sealer for 96 wells, Standard, Standard Diluent, Detection Solution A, Detection Solution B, TMB Substrate, Stop Solution, Wash Buffer (30 x concentrate), Instruction manual

Preparation and Storage

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Otoferlin

Otoferlin might be involved in the Ca(2+)-triggered synaptic vesicle-plasma membrane fusion. Mutations in the gene for Otoferlin are a cause of neurosensory nonsyndromic recessive deafness, DFNB9. The short form of the encoded protein has 3 C2 domains, a single carboxy-terminal transmembrane domain found also in the C. elegans spermatogenesis factor FER-1 and human dysferlin, while the long form has 6 C2 domains. The homology suggests that this protein may be involved in vesicle membrane fusion. Several transcript variants encoding multiple isoforms have been found for this gene.

Alternate Names

AUNB1, DFNB6, DFNB9, EC 2.7.10.1, EC 4.2.1.11, FER1L2NSRD9, Fer-1-like protein 2, otoferlin

Gene Symbol

OTOF

Additional Otoferlin Products

Product Documents for Human Otoferlin - Ready-To-Use ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Human Otoferlin - Ready-To-Use ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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