p190RhoGAP Antibody

Novus Biologicals | Catalog # NB100-88154

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all p190RhoGAP Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1200 and 1250 of human glucocorticoid receptor DNA binding factor 1 (p190RHOGAP) using the numbering given in entry EAW57452.1 (GeneID 2909).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

172 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for p190RhoGAP Antibody

Western Blot: p190RhoGAP Antibody [NB100-88154]

Western Blot: p190RhoGAP Antibody [NB100-88154]

p190RhoGAP-Antibody-Western-Blot-NB100-88154-img0008.jpg
Western Blot: p190RhoGAP Antibody [NB100-88154]

Western Blot: p190RhoGAP Antibody [NB100-88154]

Western Blot: p190RhoGAP Antibody [NB100-88154] - Whole cell lysate (50 ug) from CT26.WT, 4T1, and Renca cells. Antibodies: Affinity purified rabbit anti-GRLF1/p190RhoGAP antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Western Blot: p190RhoGAP Antibody [NB100-88154]

Western Blot: p190RhoGAP Antibody [NB100-88154]

Western Blot: p190RhoGAP Antibody [NB100-88154] - Western Blot and Immunoprecipitation: p190RhoGAP Antibody NB100-88154-Detection of Human p190RhoGAP on HeLa whole cell lysate using NB100-88154. Gp190RhoGAP was also immunoprecipitated by another rabbit ap190RhoGAP antibody.

Applications for p190RhoGAP Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 mcg/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: p190RhoGAP

The human glucocorticoid receptor DNA binding factor, which associates with the promoter region of the glucocorticoid receptor gene (hGR gene), is a repressor of glucocorticoid receptor transcription. The amino acid sequence deduced from the cDNA sequences show the presence of three sequence motifs characteristic of a zinc finger and one motif suggestive of a leucine zipper in which 1 cysteine is found instead of all leucines. The GRLF1 enhances the homologous down-regulation of wild-type hGR gene expression. Biochemical analysis suggests that GRLF1 interaction is sequence specific and that transcriptional efficacy of GRLF1 is regulated through its interaction with specific sequence motif. The level of expression is regulated by glucocorticoids. [provided by RefSeq]

Alternate Names

glucocorticoid receptor DNA binding factor 1, glucocorticoid receptor DNA-binding factor 1, glucocorticoid receptor repression factor 1, GRF1, GRF-1, GRLF1, KIAA1722, MGC10745, P190A, P190-A, p190ARhoGAP, p190RhoGAP, rho GAP p190A, Rho GTPase activating protein 35, rho GTPase-activating protein 35

Entrez Gene IDs

2909 (Human)

Gene Symbol

ARHGAP35

UniProt

Q9NRY4 (Human)

Additional p190RhoGAP Products

Product Documents for p190RhoGAP Antibody

Certificate of Analysis

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Product Specific Notices for p190RhoGAP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for p190RhoGAP Antibody

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