P2Y12/P2RY12 Antibody (HL2829)

Novus Biologicals | Catalog # NBP3-48736

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL2829
View all P2Y12/P2RY12 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain human of P2Y12/P2RY12. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for P2Y12/P2RY12 Antibody (HL2829)

P2Y12/P2RY12 Antibody (HL2829)

Immunocytochemistry/ Immunofluorescence: P2Y12/P2RY12 Antibody (HL2829) [NBP3-48736] -

P2Y12/P2RY12 antibody [HL2829] detects P2Y12/P2RY12 protein at cell membrane by immunofluorescent analysis.
Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: P2Y12/P2RY12 stained by P2Y12/P2RY12 antibody [HL2829] (NBP3-48736) diluted at 1:500.
Blue: Fluoroshield with DAPI.
P2Y12/P2RY12 Antibody (HL2829)

Western Blot: P2Y12/P2RY12 Antibody (HL2829) [NBP3-48736] -

Unboiled Non-transfected (-) and transfected (+) 293T and Expi293 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with P2Y12/P2RY12 antibody [HL2829] (NBP3-48736) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for P2Y12/P2RY12 Antibody (HL2829)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: P2Y12/P2RY12

P2Y12 is a Purinergic Receptor essential for the aggregation of blood platelets. There is evidence that mutations in P2Y12 cause a bleeding disorder, suggesting that knowledge of the P2Y12 receptor could advance the development of antiplatelet agents to treat cardiovascular diseases. Expression of P2Y12 has been reported in platelets, spinal cord, and many brain regions. ESTs for P2Y12 have been isolated from B-cell/lung/testis, brain, embryo, prostate, eye, kidney carcinoma, and colon carcinoma libraries. Cognate

Long Name

P2Y Purinergic Receptor 12

Alternate Names

ADPG-R, HORK3, P2RY12, SP1999

Gene Symbol

P2RY12

Additional P2Y12/P2RY12 Products

Product Documents for P2Y12/P2RY12 Antibody (HL2829)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for P2Y12/P2RY12 Antibody (HL2829)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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