P2Y12/P2RY12 Antibody

Novus Biologicals | Catalog # NBP1-78249

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Monkey, Primate

Cited:

Mouse, Porcine

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all P2Y12/P2RY12 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide taken within amino acid region 300-342 on human P2Y12 protein.

Reactivity Notes

Chimpanzee (100%). Porcine reactivity reported in (PMID: 31103710). Mouse reactivity reported in scientific literature (PMID:31800167).

Specificity

This antibody detects a single 56-68kDa P2Y12 diffused band. The antibody does not cross reacts with other P2X or with other P2Y receptors on western blots.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for P2Y12/P2RY12 Antibody

Western Blot: P2Y12/P2RY12 Antibody [NBP1-78249]

Western Blot: P2Y12/P2RY12 Antibody [NBP1-78249]

Western Blot: P2Y12/P2RY12 Antibody [NBP1-78249] - Antibody dilution 1:500, MW is 41 kDa
Immunocytochemistry/ Immunofluorescence: P2Y12/P2RY12 Antibody [NBP1-78249]

Immunocytochemistry/ Immunofluorescence: P2Y12/P2RY12 Antibody [NBP1-78249]

P2Y12-P2RY12-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78249-img0007.jpg
Immunohistochemistry-Paraffin: P2Y12/P2RY12 Antibody [NBP1-78249]

Immunohistochemistry-Paraffin: P2Y12/P2RY12 Antibody [NBP1-78249]

Immunohistochemistry-Paraffin: P2Y12/P2RY12 Antibody [NBP1-78249] - Mouse brain, 1:50 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxlyin QS (blue) counterstain. 40X magnification, FFPE section.
Immunohistochemistry-Paraffin: P2Y12/P2RY12 Antibody [NBP1-78249]

Immunohistochemistry-Paraffin: P2Y12/P2RY12 Antibody [NBP1-78249]

Immunohistochemistry-Paraffin: P2Y12/P2RY12 Antibody [NBP1-78249] - Rat brain, 1:100 dilution.
Immunohistochemistry-Paraffin: P2Y12/P2RY12 Antibody [NBP1-78249]

Immunohistochemistry-Paraffin: P2Y12/P2RY12 Antibody [NBP1-78249]

Immunohistochemistry-Paraffin: P2Y12/P2RY12 Antibody [NBP1-78249] - Rat spinal cord. Dilution 1:100.
Immunohistochemistry-Paraffin: P2Y12/P2RY12 Antibody [NBP1-78249]

Immunohistochemistry-Paraffin: P2Y12/P2RY12 Antibody [NBP1-78249]

Immunohistochemistry-Paraffin: P2Y12/P2RY12 Antibody [NBP1-78249] - Rat spinal cord. Dilution 1:100.
P2Y12/P2RY12 Antibody

Immunocytochemistry/ Immunofluorescence: P2Y12/P2RY12 Antibody [NBP1-78249] -

SR8278 induces microglial process extension and expression of P2Y12R and Bmal1. (a) In both the presence and absence of fA beta 1‐42, SR8278 treatment significantly induced P2Y12R and Bmal1, but not P2X7R or Per1, in a dose‐dependent manner. Synchronized BV‐2 cells were pretreated with SR8278 (20 uM) for 24 hr before treatment with fA beta 1‐42 (1 uM, 2 uM) for 2 hr. Each gene was analyzed using qPCR. *p <.05, **p <.01. (b) SR8278 (20 uM) recovered the fluorescence intensity of Bmal1 and P2Y12R and increased microglial process length in either the absence or presence of fA beta 1‐42 (P2Y12R in red and Bmal1 in green). The graph shows the average length of the longest microglial processes from the 46 microglia in each group. ***p <.001 compared to the vehicle‐treated group and ###p <.001 compared to the fA beta ‐treated group. (c) The expression of M1‐type Markers (iNOS, Cox‐2) and M2‐type markers (CD206, IL‐10, YM‐1) after SR8278 (20 uM) treatment for 24 hr in BV‐2 cells was determined using qPCR. ***p <.001 compared to the vehicle‐treated group Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31800167), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for P2Y12/P2RY12 Antibody

Application
Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

1:100

Immunoprecipitation

1:200

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris/Glycine buffer, pH 7.4-7.8, HEPES,BSA 0.5%, glycerol 30%.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: P2Y12/P2RY12

Purine nucleotide receptor are classified in to two classes, the ionotrophic ligand-gated channels, P2X and the metabotropic G-protein coupled receptors, P2Y (1). There are 7 P2X receptors and 11 P2Y (P2Y1-2, 4, 6 and 11-14) each with unique agonist response profile has been identified. The subtypes of metabotropic receptors P2Y1 and P2Y2 are predominantly expressed in arterinal smooth muscle. P2Y receptors are G protein-coupled receptors (GPCRs) activated by nucleotides, such as ATP, ADP, UTP, UDP, or UDPglucose, depending on the receptor subtype. Currently, 8 subtypes are known including P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13, and P2Y14. P2Y receptors play an important role in osteoclastic resorption and ATP and UTP are inhibitors of bone formation in osteoblasts. Activation of P2 receptors in bone cells not only involves the propagation of intercellular calcium waves but also the proliferation and activity of the cells (2). Some of these receptors tend to associate with each other to make heterodimers. ADP is an endogenous agonist for both P2Y1 and P2Y12 on platelets, and antagonism of its action at P2Y1 or P2Y12 receptor has been shown to inhibit platelet aggregation. Adenine nucleotide antagonist, INS50589, binds to P2Y12 and has been used clinically in acute coronary syndrome patients. P2Y12 is a G-protein coupled receptor characterized of presence of 7TMD. The P2Y12 is negatively coupled via Gi to adenylate cyclase. Upon activation it inhibits the adenylyl cyclase second messenger system. Stimulation of P2 receptor releases a number of factor of inflammatory mediators like IL-1b, IL-1a, IL-6, IL-8 and TNFalpha (2). The P2Y12-receptor plays a crucial role in ADP-induced platelet aggregation. Both the P2Y1 and P2Y12 receptors contribute to platelet P-selectin exposure, interactions of platelets with leukocytes and exposure of tissue factor on activated leukocytes, which should translate into a direct role of these receptors in atherosclerosis (2).The P2Y12 is highly expressed in platelets, lower levels are reported in brain and lowest levels are detected in lung, appendix, pituitary and adrenal glands. The P2Y12 is also expressed in spinal cord and fetal brain. P2Y12 receptor when expressed in host cells showed significant heterogeneity on SDS-PAGE mobility like many other PGCRs. The P2Y12 is a 342 aa protein with characteristic 7TMDs of GPCR, exhibit a broad band od 52-60kDa. The P2Y12R sequence analyses suggest the presence of putative N-glycosylation sites near the extra cellular N-terminal end of the proteins. The protein has a large 3rd intra cellular loop which interacts with G-proteins and has several phosphorylation sites. The P2Y12R-selective antibodies were generated against conserved but unique sequences on from P2Y12 protein form near C-terminal end form within 320-342 aa. The peptide sequence was conserved in human, monkey and some other species. The P2Y12R antibodies were affinity purified on an immobilized antigen based affinity chromatography. These epitope-specific antibodies strongly labeled P2Y12 protein in P2Y12-western blot positive controls. Anti-P2Y12-selective antibodies are also available as fluorescent conjugates for direct application in IHC.

Long Name

P2Y Purinergic Receptor 12

Alternate Names

ADPG-R, HORK3, P2RY12, SP1999

Gene Symbol

P2RY12

UniProt

Q9H244 (Human)

Additional P2Y12/P2RY12 Products

Product Documents for P2Y12/P2RY12 Antibody

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Product Specific Notices for P2Y12/P2RY12 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for P2Y12/P2RY12 Antibody

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