PARD6A Antibody

Novus Biologicals | Catalog # NB300-953

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all PARD6A Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-GSRIRGDGSGFSL corresponding to C-Terminus according to NP_058644.1, NP_001032358.1.

Specificity

This antibody is expected to recognise both reported isoforms (NP_058644.1; NP_001032358.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for PARD6A Antibody

Western Blot: PARD6A Antibody [NB300-953]

Western Blot: PARD6A Antibody [NB300-953]

Western Blot: PARD6A Antibody [NB300-953] - (2ug/ml) staining of Jurkat (A) and U251 (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunohistochemistry-Paraffin: PARD6A Antibody [NB300-953]

Immunohistochemistry-Paraffin: PARD6A Antibody [NB300-953]

Immunohistochemistry-Paraffin: PARD6A Antibody [NB300-953] - (10ug/ml) staining of paraffin embedded Human Human Pancreas. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
Flow Cytometry: PARD6A Antibody [NB300-953]

Flow Cytometry: PARD6A Antibody [NB300-953]

Flow Cytometry: PARD6A Antibody [NB300-953] - Analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunohistochemistry-Paraffin: PARD6A Antibody [NB300-953]

Immunohistochemistry-Paraffin: PARD6A Antibody [NB300-953]

Immunohistochemistry-Paraffin: PARD6A Antibody [NB300-953] - (5ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for PARD6A Antibody

Application
Recommended Usage

Immunohistochemistry

5 - 10 ug/ml

Immunohistochemistry-Paraffin

5 - 10 ug/ml

Peptide ELISA

Detection limit 1:8000

Western Blot

1:100 - 1:2000
Application Notes
WB: Preliminary experiments gave no signal but low background in human brain and pancreas extracts at up to 1 ug/ml. IHC-P: Human pancreas show strong cytoplasmic staining in rare cells of intralobular ducts. Human heart shows speckled staining on myocardial fibres in transverse section.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PARD6A

This gene is a member of the PAR6 family and encodes a protein with a PSD95/Discs-large/ZO1 (PDZ) domain and a semi-Cdc42/Rac interactive binding (CRIB) domain. This cell membrane protein is involved in asymmetrical cell division and cell polarization processes as a member of a multi-protein complex. The protein also has a role in the epithelial-to-mesenchymal transition (EMT) that characterizes the invasive phenotype associated with metastatic carcinomas. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Alternate Names

PAR-6, PAR-6 alpha, par-6 partitioning defective 6 homolog alpha (C. elegans), PAR6A, PAR6alpha, PAR-6Apar-6 (partitioning defective 6, C.elegans) homolog alpha, PAR6C, partitioning defective 6 homolog alpha, partitioning defective-6 homolog alpha, partitioning-defective protein 6, Tax interaction protein 40, TAX40, Tax-interacting protein 40, TIP-40PAR6

Entrez Gene IDs

50855 (Human)

Gene Symbol

PARD6A

UniProt

NP_058644. (Human)

Additional PARD6A Products

Product Documents for PARD6A Antibody

Certificate of Analysis

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Product Specific Notices for PARD6A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PARD6A Antibody

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Protocols

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FAQs

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