Park7/DJ-1 Antibody (007) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-05805

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 007 expressed in HEK293

Format

Azide and BSA Free
View all Park7/DJ-1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human Park7/DJ-1 (Uniprot#: Q99497-1; Met1-Asp189).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Park7/DJ-1 Antibody (007) - Azide and BSA Free

Park7/DJ-1 Antibody (007) - Azide and BSA Free

Flow Cytometry: Park7/DJ-1 Antibody (007) [NBP3-05805] -

Flow Cytometry: Park7/DJ-1 Antibody (007) [NBP3-05805] - Flow cytometric analysis of Human Park7/DJ-1 expression on HeLa cells. The cells were treated, stained with NBP3-05805, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Park7/DJ-1 Antibody (007) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Park7/DJ-1 Antibody (007) [NBP3-05805] -

Immunocytochemistry/ Immunofluorescence: Park7/DJ-1 Antibody (007) [NBP3-05805] - Immunofluorescence staining of Human Park7/DJ-1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP3-05805 (1:60) at 37 1 hour. Then cells were stained with the Alexa Fluor 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
Park7/DJ-1 Antibody (007) - Azide and BSA Free

Western Blot: Park7/DJ-1 Antibody (007) [NBP3-05805] -

Western Blot: Park7/DJ-1 Antibody (007) [NBP3-05805] - NBP3-05805 at 1:1000 dilution Lane A: Hela Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:20 kDa Observed band size:24 kDa
Park7/DJ-1 Antibody (007) - Azide and BSA Free

Immunoprecipitation: Park7/DJ-1 Antibody (007) [NBP3-05805] -

Immunoprecipitation: Park7/DJ-1 Antibody (007) [NBP3-05805] - PARK7 was immunoprecipitated using: Lane A:0.5 mg Jurkat Whole Cell Lysate Lane B:0.5 mg Hela Whole Cell Lysate Lane C:0.5 mg 293T Whole Cell Lysate 2 uL NBP3-05805 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-05805, at 1:1000 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 20 kDa Observed band size: 20 kDa

Applications for Park7/DJ-1 Antibody (007) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20-1:100

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:1000-1:5000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Park7/DJ-1

PARK7, also known as DJ-1, is a member of the peptidase C56 protein family. The PARK-7 protein is a positive regulator of androgen receptor-dependent transcription. It has also been reported to be a potential sensor for oxidative stress, a redox-sensitive chaperone, and a key protein for preventing neuronal oxidative stress and cell death.

PARK7 is highly expressed in pancreas, kidney, skeletal muscle, liver, testis and heart, and is detected at slightly lower levels in placenta and brain. The protein is also detected in astrocytes, Sertoli cells, spermatogonia, spermatids and spermatozoa.

Defects in this gene cause the autosomal recessive neurodegenerative disorder early-onset Parkinson's disease 7.

Long Name

Parkinson Disease 7

Alternate Names

DJ-1, DJ1

Gene Symbol

PARK7

Additional Park7/DJ-1 Products

Product Documents for Park7/DJ-1 Antibody (007) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Park7/DJ-1 Antibody (007) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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