Pax5/BSAP Antibody (PAX5/3735)

Novus Biologicals | Catalog # NBP3-07581

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Key Product Details

Species Reactivity

Human, Canine, Equine, Feline, Rhesus Macaque

Applications

Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Lambda Clone # PAX5/3735
View all Pax5/BSAP Primary Antibodies »

Product Specifications

Immunogen

A recombinant fragment (around aa 235-382) of human PAX5 protein (exact sequence is proprietary) (Uniprot: Q02548)

Reactivity Notes

Feline and Equine reactivity reported from a verified customer review.

Localization

Nuclear

Marker

Early B-Cell Marker

Specificity

The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 21,000, full-length human proteins. The PAX5 gene is a member of the paired box (PAX)family of transcription factors. The central feature of this gene family is a novel, highly conservedDNA-binding domain, known as the paired box. The PAX proteins are important regulators in early development, and alterations in the expression of their genes are thought to contribute toneoplastic transformation. The PAX5 gene encodes theB-celllineage specific activator protein (BSAP) that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis; therefore, PAX5 gene product may not only play an important role in B-cell differentiation, but also in neural development and spermatogenesis.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 Lambda

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide.

Antibody with azide - store at 2 to 8C.

Scientific Data Images for Pax5/BSAP Antibody (PAX5/3735)

Western Blot: Pax5/BSAP Antibody (PAX5/3735) [NBP3-07581]

Western Blot: Pax5/BSAP Antibody (PAX5/3735) [NBP3-07581]

Western Blot: Pax5/BSAP Antibody (PAX5/3735) [NBP3-07581] - Western Blot Analysis of Raji cell lysate using Pax5/BSAP Mouse Monoclonal Antibody (PAX5/3735).
Immunohistochemistry-Paraffin: Pax5/BSAP Antibody (PAX5/3735) [NBP3-07581]

Immunohistochemistry-Paraffin: Pax5/BSAP Antibody (PAX5/3735) [NBP3-07581]

Immunohistochemistry-Paraffin: Pax5/BSAP Antibody (PAX5/3735) [NBP3-07581] - Formalin-fixed, paraffin-embedded human Tonsil stained with Pax5/BSAP Mouse Monoclonal Antibody (Pax5/BSAP/3735).
Immunohistochemistry-Paraffin: Pax5/BSAP Antibody (PAX5/3735) [NBP3-07581]

Immunohistochemistry-Paraffin: Pax5/BSAP Antibody (PAX5/3735) [NBP3-07581]

Immunohistochemistry-Paraffin: Pax5/BSAP Antibody (PAX5/3735) [NBP3-07581] - Formalin-fixed, paraffin-embedded human Tonsil stained with Pax5/BSAP Mouse Monoclonal Antibody (Pax5/BSAP/3735).
Protein Array: Pax5/BSAP Antibody (PAX5/3735) [NBP3-07581]

Protein Array: Pax5/BSAP Antibody (PAX5/3735) [NBP3-07581]

Protein Array: Pax5/BSAP Antibody (PAX5/3735) [NBP3-07581] - Analysis of Protein Array containing more than 21,000 full-length human proteins using Pax5/BSAP Mouse Monoclonal Antibody (Pax5/BSAP/3735).
Pax5/BSAP Antibody (PAX5/3735)

Immunohistochemistry-paraffin: Mouse Monoclonal Pax5/BSAP Antibody (PAX5/3735) [NBP3-07581] -

Immunohistochemistry-paraffin: Mouse Monoclonal Pax5/BSAP Antibody (PAX5/3735) [NBP3-07581] - Pax5/BSAP immunoreactivity in a dog lymph node. Primary was used at 0.5ug/mL and was incubated with the tissue section for 30m at room temperature. Secondary was Horse Anti-Mouse HRP polymer. Image from a verified customer review.
Pax5/BSAP Antibody (PAX5/3735)

Immunohistochemistry-paraffin: Mouse Monoclonal Pax5/BSAP Antibody (PAX5/3735) [NBP3-07581] -

Immunohistochemistry-paraffin: Mouse Monoclonal Pax5/BSAP Antibody (PAX5/3735) [NBP3-07581] - Pax5/BSAP immunoreactivity in equine skin. Primary was used at 0.5ug/mL and was incubated with the tissue section for 30m at room temperature. Secondary was Horse Anti-Mouse HRP polymer. Image from a verified customer review.
Pax5/BSAP Antibody (PAX5/3735)

Immunohistochemistry-paraffin: Mouse Monoclonal Pax5/BSAP Antibody (PAX5/3735) [NBP3-07581] -

Immunohistochemistry-paraffin: Mouse Monoclonal Pax5/BSAP Antibody (PAX5/3735) [NBP3-07581] - Pax5/BSAP immunoreactivity in human tonsil. Primary was used at 0.5ug/mL and was incubated with the tissue section for 30m at room temperature. Secondary was Horse Anti-Mouse HRP polymer. Image from a verified customer review.
Pax5/BSAP Antibody (PAX5/3735)

Immunohistochemistry-paraffin: Mouse Monoclonal Pax5/BSAP Antibody (PAX5/3735) [NBP3-07581] -

Immunohistochemistry-paraffin: Mouse Monoclonal Pax5/BSAP Antibody (PAX5/3735) [NBP3-07581] - Pax5/BSAP immunoreactivity in feline intestine. Primary was used at 0.5ug/mL and was incubated with the tissue section for 30m at room temperature. Secondary was Horse Anti-Mouse HRP polymer. Image from a verified customer review.
Pax5/BSAP Antibody (PAX5/3735)

Immunohistochemistry-Frozen: Mouse Monoclonal Pax5/BSAP Antibody (PAX5/3735) [NBP3-07581] -

Immunohistochemistry-Frozen: Mouse Monoclonal Pax5/BSAP Antibody (PAX5/3735) [NBP3-07581] - Immunoreactivity was observed with this Pax5 antibody (red) in Rhesus macaque spleen tissues. Nuclei counterstained with DAPI (blue). Primary antibody dilution used: 1:100. Image from a verified customer review.
Pax5/BSAP Antibody (PAX5/3735) Immunohistochemistry-Paraffin: Pax5/BSAP Antibody (PAX5/3735) [NBP3-07581] -

Immunohistochemistry-Paraffin: Pax5/BSAP Antibody (PAX5/3735) [NBP3-07581] -

Formalin-fixed, paraffin-embedded human Tonsil stained with Pax5/BSAP Antibody (PAX5/3735).
Pax5/BSAP Antibody (PAX5/3735) Immunohistochemistry-Paraffin: Pax5/BSAP Antibody (PAX5/3735) [NBP3-07581] -

Immunohistochemistry-Paraffin: Pax5/BSAP Antibody (PAX5/3735) [NBP3-07581] -

Formalin-fixed, paraffin-embedded human Tonsil stained with Pax5/BSAP Antibody (PAX5/3735).

Applications for Pax5/BSAP Antibody (PAX5/3735)

Application
Recommended Usage

Immunohistochemistry-Frozen

Validated for Immunohistochemistry-Frozen from a verified customer review.

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Reviewed Applications

Read 5 reviews rated 5 using NBP3-07581 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Pax5/BSAP

PAX5 encodes a member of the paired box (PAX) family of transcription factors. The central feature of this gene family is a novel, highly conserved DNA-binding motif, known as the paired box. PAX proteins are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation. This gene encodes the B-cell lineage specific activator protein that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis and so the encoded protein may also play a role in neural development and spermatogenesis. This gene is located at 9p13, which is involved in t(9;14)(p13;q32) translocations recurring in small lymphocytic lymphomas of the plasmacytoid subtype, and in derived large-cell lymphomas. This translocation brings the potent E-mu enhancer of the IgH gene into close proximity of the PAX5 promoter, suggesting that the deregulation of transcription of this gene contributes to the pathogenesis of these lymphomas. Alternatively spliced transcript variants encoding different isoforms have been described but their biological validity has not been determined. [provided by RefSeq]

Long Name

Paired Box Gene 5/B-cell Specific Activator Protein

Alternate Names

BSAP

Gene Symbol

PAX5

UniProt

Q02548

Additional Pax5/BSAP Products

Product Documents for Pax5/BSAP Antibody (PAX5/3735)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Pax5/BSAP Antibody (PAX5/3735)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Pax5/BSAP Antibody (PAX5/3735) (5)

5 out of 5
5 Customer Ratings
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Customer Images

Showing  1 - 5 of 5 reviews Showing All
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  • Pax5/BSAP Antibody (PAX5/3735)
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: spleen
    Species: Rhesus Macaque
    Verified Customer | Posted 04/18/2024
    Immunoreactivity was observed with this Pax5 antibody (red) in monkey spleen tissues. Nuclei conterstained with DAPI (blue). Primary antibody dilution used: 1:100.
    Pax5/BSAP Antibody (PAX5/3735) NBP3-07581
  • Pax5/BSAP Antibody (PAX5/3735)
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Adult intestine and Intestine
    Species: Feline
    Verified Customer | Posted 10/13/2023
    Pax5 NBP3-07581 immunoreactivity in cat intestine. Primary was used at 0.5ug/mL and was incubated with the tissue section for 30m at room temperature. Secondary was Horse Anti-Mouse HRP polymer.
    Requires heat-induced epitope retrieval.
    Pax5/BSAP Antibody (PAX5/3735) NBP3-07581
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.
  • Pax5/BSAP Antibody (PAX5/3735)
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: human tonsil
    Species: Human
    Verified Customer | Posted 10/13/2023
    Pax5 NBP3-07581 immunoreactivity in human tonsil. Primary was used at 0.5ug/mL and was incubated with the tissue section for 30m at room temperature. Secondary was Horse Anti-Mouse HRP polymer.
    Pax5/BSAP Antibody (PAX5/3735) NBP3-07581
  • Pax5/BSAP Antibody (PAX5/3735)
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Skin
    Species: Equine
    Verified Customer | Posted 10/13/2023
    Pax5 (NBP3-07581) immunoreactivity in horse skin. Primary was used at 0.5ug/mL and was incubated with the tissue section for 30m at room temperature. Secondary was Horse Anti-Mouse HRP polymer.
    Pax5/BSAP Antibody (PAX5/3735) NBP3-07581
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.
  • Pax5/BSAP Antibody (PAX5/3735)
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Lymph Node
    Species: Canine
    Verified Customer | Posted 10/13/2023
    Pax5 (NBP3-07581) immunoreactivity in a dog lymph node. Primary was used at 0.5ug/mL and was incubated with the tissue section for 30m at room temperature. Secondary was Horse Anti-Mouse HRP polymer.
    Requires heat-induced epitope retrieval. I found that both citrate and EDTA buffer works, but the signal is better with EDTA.
    Pax5/BSAP Antibody (PAX5/3735) NBP3-07581

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