PAX8 Antibody

Western Blot: PAX8 Antibody [NBP2-29907]

Western Blot: PAX8 Antibody [NBP2-29907] - Analysis of 60ug of COS7 whole cell lysate (left lane), negative control HeLa lysate (right lane), and 10ul PageRuler Prestained Protein Ladder.

Western Blot: PAX8 Antibody [NBP2-29907]

Western Blot: PAX8 Antibody [NBP2-29907] - Analysis of 20ug of the indicated whole cell lysates onto a 4-12% Novex Bis-Tris polyacrylamide gel. Data courtesy of the Innovators Program.

Immunocytochemistry/ Immunofluorescence: PAX8 Antibody [NBP2-29907]

Immunocytochemistry/Immunofluorescence: PAX8 Antibody [NBP2-29907] - Analysis of PAX8 (green) in 293T (left panel) and COS7 cells (right panel). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with a PAX8 polyclonal antibody at a dilution of 1:400 for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight-554 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.

Immunoprecipitation: PAX8 Antibody [NBP2-29907]

Immunoprecipitation: PAX8 Antibody [NBP2-29907] - Analysis of PAX8 was performed using COS7 whole cell lysate. Antigen-antibody complexes were formed by incubating 500ug of lysate with 3ug of a PAX8 polyclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Agarose, washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer. Samples was resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween-20 for 1 hour. The membrane was probed with a PAX8 polyclonal antibody at a dilution of 1:1000 overnight rotating at 4C. Membranes were washed in TBST, and probed with Clean-Blot IP Detection Reagent at a dilution of 1:1000 for at least one hour. Chemiluminescent detection was performed using SuperSignal West Pico.