PCNA Antibody (SY12-07)

Novus Biologicals | Catalog # NBP2-67390

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SY12-07 expressed in HEK293
View all PCNA Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human PCNA aa 88-137 / 261. (SwissProt: P12004 Human; SwissProt: P17918 Mouse; SwissProt: P04961 Rat)

Localization

Nucleus.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PCNA Antibody (SY12-07)

Western Blot: PCNA Antibody (SY12-07) [NBP2-67390]

Western Blot: PCNA Antibody (SY12-07) [NBP2-67390]

Western Blot: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of PCNA on L6 cell lysates with Rabbit anti-PCNA antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 29 kDa Observed band size: 36 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.
Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390]

Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390]

Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of paraffin-embedded mouse stomach tissue using anti-PCNA antibody. Counter stained with hematoxylin.
Flow Cytometry: PCNA Antibody (SY12-07) [NBP2-67390]

Flow Cytometry: PCNA Antibody (SY12-07) [NBP2-67390]

Flow Cytometry: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of Hela cells with PCNA antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western Blot: PCNA Antibody (SY12-07) [NBP2-67390]

Western Blot: PCNA Antibody (SY12-07) [NBP2-67390]

Western Blot: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of PCNA on different lysates using anti-PCNA antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: 293 Lane 3: A431
Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390]

Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390]

Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of paraffin-embedded human breast carcinama tissue using anti-PCNA antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390]

Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390]

Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of paraffin-embedded human spleen tissue using anti-PCNA antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390]

Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390]

Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of paraffin-embedded human tonsil tissue using anti-PCNA antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390]

Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390]

Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of paraffin-embedded mouse colon tissue using anti-PCNA antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390]

Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390]

Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of paraffin-embedded mouse spleen tissue using anti-PCNA antibody. Counter stained with hematoxylin.
PCNA Antibody (SY12-07) Western Blot: PCNA Antibody (SY12-07) [NBP2-67390]

Western Blot: PCNA Antibody (SY12-07) [NBP2-67390]

Western blot analysis on different lysates with NBP2-67390 at 1/1,000 dilution. Lane 1: Mouse skin tissue lysate Lane 2: Mouse spleen tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 29 kDa Observed band size: 34 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-67390 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Applications for PCNA Antibody (SY12-07)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry-Paraffin

1:100-1:500

Western Blot

1:1000-1:5000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PCNA

Proliferating Cell Nuclear Antigen (PCNA) is a DNA polymerase that is essential for DNA replication, excision and mismatch repair pathways. PCNA is a "DNA clamp" which binds to the CDK inhibitor p21, the structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT). PCNA is a potentially useful marker of cells with proliferative potential and for identifying the proliferation status of tumor tissue (i.e. relevant to prognosis). PCNA antibodies are also useful tools for DNA repair and cancer research.

Long Name

Proliferating Cell Nuclear Antigen

Alternate Names

cyclin, DNA polymerase delta auxiliary protein, MGC8367, proliferating cell nuclear antigen

Gene Symbol

PCNA

Additional PCNA Products

Product Documents for PCNA Antibody (SY12-07)

Certificate of Analysis

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Product Specific Notices for PCNA Antibody (SY12-07)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PCNA Antibody (SY12-07)

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Protocols

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