PCP4 Antibody (CL5306) - BSA Free

Novus Biologicals | Catalog # NBP2-61410

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL5306

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P48539, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for PCP4 Antibody (CL5306) - BSA Free

PCP4 Antibody (CL5306) - BSA Free Immunohistochemistry: PCP4 Antibody (CL5306) [NBP3-52479]

Immunohistochemistry: PCP4 Antibody (CL5306) [NBP3-52479]

Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
PCP4 Antibody (CL5306) - BSA Free Immunohistochemistry: PCP4 Antibody (CL5306) [NBP3-52479]

Immunohistochemistry: PCP4 Antibody (CL5306) [NBP3-52479]

Staining of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.
PCP4 Antibody (CL5306) - BSA Free Immunohistochemistry: PCP4 Antibody (CL5306) [NBP3-52479]

Immunohistochemistry: PCP4 Antibody (CL5306) [NBP3-52479]

Staining of human testis shows no positivity in cells in seminiferous ducts as expected.
PCP4 Antibody (CL5306) - BSA Free Immunohistochemistry: PCP4 Antibody (CL5306) [NBP3-52479]

Immunohistochemistry: PCP4 Antibody (CL5306) [NBP3-52479]

Staining of human tonsil shows no positivity in non-germinal center cells as expected.
PCP4 Antibody (CL5306) - BSA Free Immunohistochemistry: PCP4 Antibody (CL5306) [NBP3-52479]

Immunohistochemistry: PCP4 Antibody (CL5306) [NBP3-52479]

Staining of mouse brain shows strong cytoplasmic positivity in Purkinje cells.
PCP4 Antibody (CL5306) - BSA Free Immunohistochemistry: PCP4 Antibody (CL5306) [NBP3-52479]

Immunohistochemistry: PCP4 Antibody (CL5306) [NBP3-52479]

Staining of rat brain shows strong cytoplasmic positivity in Purkinje cells.

Applications for PCP4 Antibody (CL5306) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reviewed Applications

Read 1 review rated 3 using NBP2-61410 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PCP4

Alternate Names

brain specific polypeptide PEP19, 10Brain-specific antigen PCP-4, Brain-specific polypeptide PEP-19, PEP19, PEP-19, Purkinje cell protein 4

Gene Symbol

PCP4

Additional PCP4 Products

Product Documents for PCP4 Antibody (CL5306) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for PCP4 Antibody (CL5306) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PCP4 Antibody (CL5306) - BSA Free

Customer Reviews for PCP4 Antibody (CL5306) - BSA Free (1)

3 out of 5
1 Customer Rating
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Customer Images

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  • PCP4 Antibody (CL5306)
    Name: Anonymous
    Application: IHC – Free floating
    Sample Tested: Cerebellum and hippocampal tissue sections
    Species: Rat
    Verified Customer | Posted 07/25/2018
    IF staining of rat cerebellum (A) and rat hippocampal CA2 region (B) with NBP2-61410 (red, left) and a polyclonal PCP4 antibody (green, middle). The panels on the right show the merged red and green channels. Staining was carried out without (upper row) or with antigen retrieval.
    PCP4 Antibody (CL5306) - BSA Free NBP2-61410

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Protocols

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FAQs

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