PDE3A Antibody

Novus Biologicals | Catalog # NBP2-19744

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all PDE3A Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human PDE3A. The exact sequence is proprietary.

Reactivity Notes

Pig (88%). Rat reactivity reported from a verified customer review.

Localization

Membrane; Peripheral membrane protein

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

125 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PDE3A Antibody

Western Blot: PDE3A Antibody [NBP2-19744]

Western Blot: PDE3A Antibody [NBP2-19744]

Western Blot: PDE3A Antibody [NBP2-19744] - Antibody specificity is verified in 293T cells with siPDE3A knockdown (first 6 lanes with varying siRNA concentrations). 5 nM and 25 nM resulted in ~30% protein reduction (lanes 2, 3 vs lanes 5, 6). Loading of all 293T cell lysates are ~30 ug. H9C2 lysate is ~7 ug due to limited materials. Antibody at 1:500. Secondary antibody at 1:10,000. Blocked with 5% BSA. WB image submitted by a verified customer review.
Western Blot: PDE3A Antibody [NBP2-19744]

Western Blot: PDE3A Antibody [NBP2-19744]

Western Blot: PDE3A Antibody [NBP2-19744] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 5% SDS PAGE gel, diluted at 1:1000.

Applications for PDE3A Antibody

Application
Recommended Usage

Western Blot

1:500 - 1:3000

Reviewed Applications

Read 1 review rated 4 using NBP2-19744 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PDE3A

Cyclic nucleotide phosphodiesterases (PDEs) comprise a complex group of enzymes, and at least 5 major PDE families or classes with distinctive properties have been identified. Members of the cGMP-inhibited cAMP PDE (cGI-PDE) family, such as PDE3A, are characterized by high affinity for cAMP and cGMP and competitive inhibition of their cAMP hydrolytic activity by cGMP and certain positive inotropic agents (Meacci et al., 1992 [PubMed 1315035]).[supplied by OMIM]

Long Name

cGMP-inhibited 3',5'-cyclic phosphodiesterase A

Alternate Names

cGI-PDE, CGI-PDE A

Gene Symbol

PDE3A

UniProt

Q14432 (Human)

Additional PDE3A Products

Product Documents for PDE3A Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for PDE3A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Customer Reviews for PDE3A Antibody (1)

4 out of 5
1 Customer Rating
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  • PDE3A Antibody
    Name: YINZHOU ZHU
    Application: Western Blot
    Sample Tested: 293T lysates and H9c2 whole cell lysate
    Species: Human and Rat
    Verified Customer | Posted 01/10/2020
    Loading of all 293T cell lysates are ~30ug. H9C2 lysate is ~7ug due to limited materials.
    Antibody specificity is verified in 293T cells with siPDE3A knockdown (first 6 lanes with varying siRNA concentrations). 5nM and 25nM resulted in ~30% protein reduction (lanes 2, 3 vs lanes 5, 6). Ab dilution: 1:1500 Secondary incubation: 1:10000 Blocking: 5% BSA
    PDE3A Antibody NBP2-19744

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