PDZK1 Antibody - BSA Free

Novus Biologicals | Catalog # NB400-149

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Bovine, Rat (Negative)

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PDZK1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to the C-terminus of human PDZK1. [UniProt# Q13113]

Reactivity Notes

Does not react with rat.

Localization

Cytoplasmic and associated with peripheral membranes; localizes to the apical compartment of proximal tubular cells and to sinusoidal liver membranes.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PDZK1 Antibody - BSA Free

Western Blot: PDZK1 Antibody [NB400-149]

Western Blot: PDZK1 Antibody [NB400-149]

Western Blot: PDZK1 Antibody [NB400-149] - WB analysis of PDZK1 in fetal human liver lysate.
Immunocytochemistry/ Immunofluorescence: PDZK1 Antibody [NB400-149]

Immunocytochemistry/ Immunofluorescence: PDZK1 Antibody [NB400-149]

Immunocytochemistry/Immunofluorescence: PDZK1 Antibody [NB400-149] - PDZK1 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).
Immunohistochemistry: PDZK1 Antibody [NB400-149]

Immunohistochemistry: PDZK1 Antibody [NB400-149]

Immunohistochemistry: PDZK1 Antibody [NB400-149] - IHC analysis of PDZK1 in mouse kidney using DAB with hematoxylin counterstain.
Simple Western: PDZK1 Antibody [NB400-149]

Simple Western: PDZK1 Antibody [NB400-149]

Simple Western: PDZK1 Antibody [NB400-149] - Simple Western lane view shows a specific band for PDZK1 in 0.2 mg/ml of Human Liver lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. *Non-specific interaction with the 230 kDa standard may be seen with this antibody.

Applications for PDZK1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:200

Immunohistochemistry

1:400

Immunohistochemistry-Paraffin

1:400

Simple Western

1:250

Western Blot

1:1000
Application Notes
In Western blot a single band is observed at approx. 70 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in Human Liver lysate 0.2 mg/mL, separated by Size, antibody dilution of 1:250. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PDZK1

Scaffold or adaptor proteins recruits and/or anchor their binding partners to specific subcellular locations, serving as a platform to regulate interactions between proteins involved in diverse signal transduction pathways, and PDZK1 (PDZ domain-containing protein 1) is a scaffold protein belonging to NHERFs family. PDZK1 expression is largely limited to epithelial cells and is mainly expressed in renal proximal epithelial cells, hepatocytes, and at a lower level in other epithelial as well as certain endothelial cells. PDZK1 has four PDZ domains which facilitates its interaction with its binding partners, including ion transporters (e.g. CFTR, OCTN1/OCTN2 etc) and several GPCRs (e.g. HTR2B, SSTR family members). Through its interaction with HDL and SR-BI, PDZK1 can play role in reverse cholesterol transport and for HDL-mediated vascular re-endothelialisation. When complex with SLC9A3R1, PDZK1 cluster proteins that are functionally dependent in a mutual fashion and modulate the trafficking as well as the activity of associated membrane proteins. PDZK1 plays a role in cellular mechanisms associated with multidrug resistance through its interaction with ABCC2 and PDZK1IP1. PDZK1 also implicates in connecting SCARB1 with cellular machineries for intracellular cholesterol transport and/or metabolism and regulation of proximal tubular Na+-dependent inorganic phosphate cotransport.

Long Name

PDZ Domain Containing 1

Alternate Names

CAP70, CLAMP, NaPi-Cap1, NHERF3, PDZD1

Entrez Gene IDs

5174 (Human); 59020 (Mouse); 65144 (Rat)

Gene Symbol

PDZK1

UniProt

Q5T2W1

Additional PDZK1 Products

Product Documents for PDZK1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PDZK1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epithelial Cell Polarity

Citations for PDZK1 Antibody - BSA Free

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Protocols

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