PEPD Antibody (OTI1B7)

Novus Biologicals | Catalog # NBP3-44639

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1B7
View all PEPD Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human PEPD (NP_000276) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for PEPD Antibody (OTI1B7)

PEPD Antibody (OTI1B7) Immunohistochemistry;PEPD Antibody (OTI1B7)[NBP3-44639]-

Immunohistochemistry;PEPD Antibody (OTI1B7)[NBP3-44639]-

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PEPD mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.
PEPD Antibody (OTI1B7) Immunocytochemistry/ Immunofluorescence;PEPD Antibody (OTI1B7)[NBP3-44639]-

Immunocytochemistry/ Immunofluorescence;PEPD Antibody (OTI1B7)[NBP3-44639]-

Anti-PEPD mouse monoclonal antibody immunofluoreaining of COS7 cells transiently transfected by pCMV6-ENTRY PEPD.
PEPD Antibody (OTI1B7) Immunohistochemistry;PEPD Antibody (OTI1B7)[NBP3-44639]-

Immunohistochemistry;PEPD Antibody (OTI1B7)[NBP3-44639]-

Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PEPD mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.
PEPD Antibody (OTI1B7) Western Blot;PEPD Antibody (OTI1B7)[NBP3-44639]-

Western Blot;PEPD Antibody (OTI1B7)[NBP3-44639]-

Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-PEPD monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
PEPD Antibody (OTI1B7) Immunohistochemistry;PEPD Antibody (OTI1B7)[NBP3-44639]-

Immunohistochemistry;PEPD Antibody (OTI1B7)[NBP3-44639]-

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PEPD mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.
PEPD Antibody (OTI1B7) Western Blot;PEPD Antibody (OTI1B7)[NBP3-44639]-

Western Blot;PEPD Antibody (OTI1B7)[NBP3-44639]-

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PEPD (, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PEPD.
PEPD Antibody (OTI1B7) Western Blot;PEPD Antibody (OTI1B7)[NBP3-44639]-

Western Blot;PEPD Antibody (OTI1B7)[NBP3-44639]-

Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-PEPD monoclonal antibody (1:200).
PEPD Antibody (OTI1B7) Immunohistochemistry;PEPD Antibody (OTI1B7)[NBP3-44639]-

Immunohistochemistry;PEPD Antibody (OTI1B7)[NBP3-44639]-

Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-PEPD mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.
PEPD Antibody (OTI1B7) Flow Cytometry;PEPD Antibody (OTI1B7)[NBP3-44639]-

Flow Cytometry;PEPD Antibody (OTI1B7)[NBP3-44639]-

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PEPD antibody, and then analyzed by flow cytometry.
PEPD Antibody (OTI1B7) Immunohistochemistry;PEPD Antibody (OTI1B7)[NBP3-44639]-

Immunohistochemistry;PEPD Antibody (OTI1B7)[NBP3-44639]-

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PEPD mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.
PEPD Antibody (OTI1B7) Immunohistochemistry;PEPD Antibody (OTI1B7)[NBP3-44639]-

Immunohistochemistry;PEPD Antibody (OTI1B7)[NBP3-44639]-

Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-PEPD mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Applications for PEPD Antibody (OTI1B7)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:2000

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

>95%

Reconstitution

For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized powder ( 1X PBS, pH 7.3,)

Preservative

8% Trehalose

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 degrees C. Avoid freeze/thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PEPD

The PEPD gene encodes a member of the peptidase family. The protein forms a homodimer that hydrolyzes dipeptides or tripeptides with C-terminal proline or hydroxyproline residues. The enzyme serves an important role in the recycling of proline, and may be rat

Alternate Names

aminoacyl-L-proline hydrolase, EC 3.4.13.9, Imidodipeptidase, MGC10905, peptidase Dimidodipeptidase, PRD, PROLIDASE, Proline dipeptidase, xaa-Pro dipeptidase, X-Pro dipeptidase

Gene Symbol

PEPD

Additional PEPD Products

Product Documents for PEPD Antibody (OTI1B7)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for PEPD Antibody (OTI1B7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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