Phosphothreonine Antibody [HRP]

Novus Biologicals | Catalog # NBP1-77696

Novus Biologicals

Key Product Details

Validated by

Biological Validation

Species Reactivity

Non-species specific

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunoprecipitation

Label

HRP

Antibody Source

Polyclonal Rabbit IgG
View all Phosphothreonine Primary Antibodies »

Product Specifications

Immunogen

Phosphothreonine Antibody was prepared from whole rabbit serum produced by repeated immunizations with phosphothreonine conjugated KLH.

Reactivity Notes

Reactivity is specific for phosphothreonine and minimal cross reactivity is observed against phosphotyrosine or phosphoserine

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room

This product was prepared from monospecific antiserum by immunoaffinity chromatography using phosphorylated threonine peptide coupled to agarose followed by conjugation to horseradish peroxidase and subsequent purification. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG and anti-Peroxidase

Scientific Data Images for Phosphothreonine Antibody [HRP]

Western Blot: Phosphothreonine Antibody [HRP] [NBP1-77696] - Affinity Purified Detection phosphorylated proteins present in mouse spleen cell lysates. Left Lane: extract of spleen from mouse treated with vanadium. Right lane: extract of spleen from untreated mouse.
Phosphothreonine Antibody [HRP]

Phosphothreonine Antibody [HRP]

Affinity Purified anti-phosphothreonine (pT) antibody is shown to detect phosphorylated proteins present in mouse spleen cell lysates. Left Lane: extract of spleen from mouse treated with vanadium. Right lane: extract of spleen from untreated mouse.

Applications for Phosphothreonine Antibody [HRP]

Application
Recommended Usage

ELISA

1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:500
Application Notes
This product is suitable for use in ELISA and western blotting assays. The antibody reacts specifically with phosphothreonine and shows minimal reactivity by ELISA and western blotting with proteins containing phosphotyrosine or phosphoserine residues. The antibody reacts with free phosphothreonine, phosphothreonine conjugated to carriers such as thyroglobulin or BSA, and detects the presence of phosphothreonine proteins in both unstimulated and stimulated cell lysates. Although not tested, this antibody is likely functional in RIA, flow cytometry and immunohistochemistry.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 50% (v/v) Glycerol

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Phosphothreonine

Changes in the serine/threonine phosphorylation state of a protein in response to various external stimuli can have profound effects on cellular signal transduction, apoptosis and carcinogenesis. The reagents, including phosphorylated protein/peptides, antibodies against the phosphospecific amino acid, are important tools to explore the activation of serine, threonine or tyrosine containing proteins. An aberrant protein phosphorylation is a hallmark of human disease, and the enzymes, particularly protein kinases, which control protein phosphorylation are recognized as a major new drug target family.

Alternate Names

C4H10NO6P;O-phospho-L-threonine;Phospho-threonine;pThr;Threonine

Additional Phosphothreonine Products

Product Documents for Phosphothreonine Antibody [HRP]

Certificate of Analysis

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Product Specific Notices for Phosphothreonine Antibody [HRP]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Phosphothreonine Antibody [HRP]

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Protocols

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FAQs

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