PKC beta Antibody

Novus Biologicals | Catalog # NBP2-19846

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Rat, Xenopus

Cited:

Human, Rat

Predicted:

Bovine (97%), Mouse (98%), Rabbit (98%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Functional Assay

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all PKC beta Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human PKC beta. The exact sequence is proprietary.

Localization

Cytoplasm (By similarity); Membrane; Peripheral membrane protein

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

77 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PKC beta Antibody

Immunohistochemistry-Paraffin: PKC beta Antibody [NBP2-19846]

Immunohistochemistry-Paraffin: PKC beta Antibody [NBP2-19846]

Immunohistochemistry-Paraffin: PKC beta Antibody [NBP2-19846] - Rat brain. PKC beta antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: PKC beta Antibody [NBP2-19846]

Western Blot: PKC beta Antibody [NBP2-19846]

Western Blot: PKC beta Antibody [NBP2-19846] - Human neuroblastoma cells untreated or treated with various drugs. Image submitted by a verified customer review.
PKC beta Antibody

Immunocytochemistry/ Immunofluorescence: PKC beta Antibody [NBP2-19846] -

Immunocytochemistry/ Immunofluorescence: PKC beta Antibody [NBP2-19846] - PKC beta antibody detects PKC beta protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: SK-N-AS cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PKC beta stained by PKC beta antibody (NBP2-19846) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
PKC beta Antibody

Western Blot: PKC beta Antibody [NBP2-19846] -

Western Blot: PKC beta Antibody [NBP2-19846] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC beta antibody (NBP2-19846) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for PKC beta Antibody

Application
Recommended Usage

Functional Assay

Assay dependent

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PKC beta

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq]

Alternate Names

EC 2.7.11, EC 2.7.11.13, MGC41878, PKC-B, PKC-beta, PKCBPRKCB2, PRKCB1protein kinase C beta 1, protein kinase C beta type, protein kinase C, beta, protein kinase C, beta 1, protein kinase C, beta 1 polypeptide

Gene Symbol

PRKCB

UniProt

P05771

Additional PKC beta Products

Product Documents for PKC beta Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for PKC beta Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for PKC beta Antibody

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Protocols

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FAQs

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