PLD1 Antibody (2F3) - Azide and BSA Free

Novus Biologicals | Catalog # H00005337-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2F3

Format

Azide and BSA Free
View all PLD1 Primary Antibodies »

Product Specifications

Immunogen

PLD1 (NP_002653, 965 a.a. ~ 1074 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GYLDDPSEDIQDPVSDKFFKEVWVSTAARNATIYDKVFRCLPNDEVHNLIQLRDFINKPVLAKEDPIRAEEELKKIRGFLVQFPFYFLSEESLLPSVGTKEAIVPMEVWT

Specificity

PLD1 - phospholipase D1, phophatidylcholine-specific

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PLD1 Antibody (2F3) - Azide and BSA Free

Western Blot: PLD1 Antibody (2F3) [H00005337-M01]

Western Blot: PLD1 Antibody (2F3) [H00005337-M01]

Western Blot: PLD1 Antibody (2F3) [H00005337-M01] - Analysis of PLD1 expression in transfected 293T cell line by PLD1 monoclonal antibody (M01), clone 2F3.Lane 1: PLD1 transfected lysate(124.2 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: PLD1 Antibody (2F3) [H00005337-M01] - Detection limit for recombinant GST tagged PLD1 is 0.03 ng/ml as a capture antibody.

Applications for PLD1 Antibody (2F3) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PLD1

Phosphatidylcholine (PC)-specific phospholipases D (PLDs; EC 3.1.4.4) catalyze the hydrolysis of PC to produce phosphatidic acid and choline. A range of agonists acting through G protein-coupled receptors and receptor tyrosine kinases stimulate this hydrolysis. PC-specific PLD activity has been implicated in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis (Hammond et al., 1995 [PubMed 8530346]).[supplied by OMIM]

Long Name

Phospholipase D1

Alternate Names

Choline phosphatase 1

Entrez Gene IDs

5337 (Human)

Gene Symbol

PLD1

OMIM

602382 (Human)

UniProt

NP_002653 (Human)

Additional PLD1 Products

Product Documents for PLD1 Antibody (2F3) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for PLD1 Antibody (2F3) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PLD1 Antibody (2F3) - Azide and BSA Free

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