PLZF Antibody (3A7) - Azide and BSA Free

Novus Biologicals | Catalog # H00007704-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Proximity Ligation Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3A7

Format

Azide and BSA Free
View all PLZF Primary Antibodies »

Product Specifications

Immunogen

ZBTB16 (AAH29812, 381 a.a. ~ 480 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. QRELFSKLGELAVGMKSESRTIGEQCSVCGVELPDNEAVEQHRKLHSGMKTYGCELCGKRFLDSLRLRMHLLAHSAGAKAFVCDQCGAQFSKEDALETHR

Localization

Nuclear

Specificity

ZBTB16 - zinc finger and BTB domain containing 16

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PLZF Antibody (3A7) - Azide and BSA Free

Western Blot: PLZF Antibody (3A7) [H00007704-M01]

Western Blot: PLZF Antibody (3A7) [H00007704-M01]

Western Blot: PLZF Antibody (3A7) [H00007704-M01] - Analysis of ZBTB16 expression in transfected 293T cell line by ZBTB16 monoclonal antibody (M01), clone 3A7.Lane 1: ZBTB16 transfected lysate (Predicted MW: 11.11 KDa).Lane 2: Non-transfected lysate.
Proximity Ligation Assay: PLZF Antibody (3A7) [H00007704-M01]

Proximity Ligation Assay: PLZF Antibody (3A7) [H00007704-M01]

Proximity Ligation Assay: PLZF Antibody (3A7) [H00007704-M01] - Analysis of protein-protein interactions between TRAF2 and ZBTB16. HeLa cells were stained with anti-TRAF2 rabbit purified polyclonal 1:1200 and anti-ZBTB16 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Sandwich ELISA: PLZF Antibody (3A7) [H00007704-M01] - Detection limit for recombinant GST tagged ZBTB16 is approximately 1ng/ml as a capture antibody.

Applications for PLZF Antibody (3A7) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Proximity Ligation Assay

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against recombinant protein on ELISA. GST alone used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PLZF

This gene is a member of the Krueppel C2H2-type zinc-finger protein family and encodes a zinc finger transcription factor that contains nine Kruppel-type zinc finger domains at the carboxyl terminus. This protein is located in the nucleus, is involved in cell cycle progression, and interacts with a histone deacetylase. Specific instances of aberrant gene rearrangement at this locus have been associated with acute promyelocytic leukemia (APL). Alternate transcriptional splice variants have been characterized.

Long Name

Promyelocytic Leukemia Zinc Finger

Alternate Names

ZBTB16, ZNF145

Entrez Gene IDs

7704 (Human)

Gene Symbol

ZBTB16

OMIM

176797 (Human)

UniProt

AAH29812 (Human)

Additional PLZF Products

Product Documents for PLZF Antibody (3A7) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for PLZF Antibody (3A7) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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