POU3F2/OCT7 Antibody

Novus Biologicals | Catalog # NBP2-21585

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Chimpanzee

Cited:

Mouse

Predicted:

Bovine (100%), Xenopus (97%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all POU3F2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human POU3F2/OCT7. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for POU3F2/OCT7 Antibody

Chromatin Immunoprecipitation: POU3F2/OCT7 Antibody [NBP2-21585] - Sample: 293T whole cell lysate/extract A. 5 ug preimmune rabbit IgG B. 5 ug of OCT7 antibody The precipitated DNA was detected by PCR with primer set targeting to MITF promoter.
Immunohistochemistry-Paraffin: POU3F2/OCT7 Antibody [NBP2-21585]

Immunohistochemistry-Paraffin: POU3F2/OCT7 Antibody [NBP2-21585]

Immunohistochemistry-Paraffin: POU3F2/OCT7 Antibody [NBP2-21585] - TOV-21G xenograft, using Brn2 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunoprecipitation: POU3F2/OCT7 Antibody [NBP2-21585]

Immunoprecipitation: POU3F2/OCT7 Antibody [NBP2-21585]

Immunoprecipitation: POU3F2/OCT7 Antibody [NBP2-21585] - Sample: 1000 ug 293T whole cell lysate/extract A. 50 ug 293T whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of OCT7 protein by 2 ug of Brn2 antibody 10% SDS-PAGE The immunoprecipitated OCT7 protein was detected by OCT7. antibody diluted at 1:1000. anti-rabbit IgG was used as a secondary reagent.
POU3F2/OCT7 Antibody

Western Blot: POU3F2/OCT7 Antibody [NBP2-21585] -

Western Blot: POU3F2/OCT7 Antibody [NBP2-21585] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with POU3F2/OCT7 antibody (NBP2-21585) diluted at 1:1000.
POU3F2/OCT7 Antibody

Immunohistochemistry-Frozen: POU3F2/OCT7 Antibody [NBP2-21585] -

Brn2 antibody detects Brn2 protein expression at nucleus by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: Brn2 protein stained by Brn2 antibody (NBP2-21585) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500.
Blue: Fluoroshield with DAPI.
POU3F2/OCT7 Antibody

Immunohistochemistry-Paraffin: POU3F2/OCT7 Antibody [NBP2-21585] -

Brn2 antibody detects Brn2 protein at nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Brn2 antibody (NBP2-21585) diluted at 1:500.
br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
POU3F2/OCT7 Antibody

Immunocytochemistry/ Immunofluorescence: POU3F2/OCT7 Antibody [NBP2-21585] -

Brn2 antibody detects Brn2 protein at nucleus by immunofluorescent analysis.
Sample: SK-N-AS cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Brn2 protein stained by Brn2 antibody (NBP2-21585) diluted at 1:1000.
Red: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.
Scale bar = 10 um.
POU3F2/OCT7 Antibody

Immunohistochemistry-Paraffin: POU3F2/OCT7 Antibody [NBP2-21585] -

Brn2 antibody detects Brn2 protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Brn2 antibody (NBP2-21585) diluted at 1:500.
br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for POU3F2/OCT7 Antibody

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

Assay-dependent

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: POU3F2

POU3F2 belongs to a large family of transcription factors that bind to the octameric DNA sequence ATGCAAAT. Most of these proteins share a highly homologous region, referred to as the POU domain, that occurs in several mammalian transcription factors, including the octamer-binding proteins Oct1 (POU2F1; MIM 164175) and Oct2 (POU2F2; MIM 164176) and the pituitary protein Pit1 (PIT1; MIM 173110). Class III POU genes are expressed predominantly in the central nervous system (CNS). It is likely that CNS-specific transcription factors such as these play an important role in mammalian neurogenesis by regulating their diverse patterns of gene expression (Schreiber et al., 1993 [PubMed 8441633]; Atanasoski et al., 1995 [PubMed 7601453]).[supplied by OMIM]

Long Name

POU Class 3 Homeobox 2

Alternate Names

Brain-2, BRN2, Oct7, OTF7

Gene Symbol

POU3F2

UniProt

P20265

Additional POU3F2 Products

Product Documents for POU3F2/OCT7 Antibody

Certificate of Analysis

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Product Specific Notices for POU3F2/OCT7 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for POU3F2/OCT7 Antibody

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Protocols

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FAQs

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