PP2C beta/PPM1B Antibody (1A3-2A4) - Azide and BSA Free

Novus Biologicals | Catalog # H00005495-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Proximity Ligation Assay, Knockdown Validated

Cited:

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1A3-2A4

Format

Azide and BSA Free
View all PP2C beta/PPM1B Primary Antibodies »

Product Specifications

Immunogen

PPM1B (AAH12002, 1 a.a. ~ 192 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSIVLVCFSNAPKVSDEAVKKDSELDKHLESRVEEIMEKSGEEGMPDLAHVMRILSAENIPNLPPGGGLAGKRNVIEAVYSRLNPHRESDGASDEAEESGSQGKLVEALRQMRINHRGNYRQLLEEMLTSYRLAKVEGEESPAEPAATATSSNSDAGNPVTMQESHTESESGLAELDSSNEDAGTKMSGEKI

Specificity

PPM1B - protein phosphatase 1B (formerly 2C), magnesium-dependent, beta isoform

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PP2C beta/PPM1B Antibody (1A3-2A4) - Azide and BSA Free

Western Blot: PP2C beta/PPM1B Antibody (1A3-2A4) [H00005495-M01]

Western Blot: PP2C beta/PPM1B Antibody (1A3-2A4) [H00005495-M01]

Western Blot: PP2C beta/PPM1B Antibody (1A3-2A4) [H00005495-M01] - Analysis of PPM1B expression in transfected 293T cell line by PPM1B monoclonal antibody (M01), clone 1A3-2A4.Lane 1: PPM1B transfected lysate(52.643 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: PP2C beta/PPM1B Antibody (1A3-2A4) [H00005495-M01]

Immunocytochemistry/ Immunofluorescence: PP2C beta/PPM1B Antibody (1A3-2A4) [H00005495-M01]

Immunocytochemistry/Immunofluorescence: PP2C beta/PPM1B Antibody (1A3-2A4) [H00005495-M01] - Analysis of monoclonal antibody to PPM1B on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: PP2C beta/PPM1B Antibody (1A3-2A4) [H00005495-M01]

Western Blot: PP2C beta/PPM1B Antibody (1A3-2A4) [H00005495-M01]

Western Blot: PP2C beta/PPM1B Antibody (1A3-2A4) [H00005495-M01] - Analysis of PPM1B over-expressed 293 cell line, cotransfected with PPM1B Validated Chimera RNAi ( Cat # H00005495-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PPM1B monoclonal antibody (M01), clone 1A3-2A4 (Cat # H00005495-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Proximity Ligation Assay: PP2C beta/PPM1B Antibody (1A3-2A4) [H00005495-M01]

Proximity Ligation Assay: PP2C beta/PPM1B Antibody (1A3-2A4) [H00005495-M01]

Proximity Ligation Assay: PP2C beta/PPM1B Antibody (1A3-2A4) [H00005495-M01] - Analysis of protein-protein interactions between ARRB2 and PPM1B. HeLa cells were stained with anti-ARRB2 rabbit purified polyclonal 1:1200 and anti-PPM1B mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Immunoprecipitation: PP2C beta/PPM1B Antibody (1A3-2A4) [H00005495-M01]

Immunoprecipitation: PP2C beta/PPM1B Antibody (1A3-2A4) [H00005495-M01]

Immunoprecipitation: PP2C beta/PPM1B Antibody (1A3-2A4) [H00005495-M01] - Analysis of PPM1B transfected lysate using anti-PPM1B monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PPM1B monoclonal antibody.
ELISA: PP2C beta/PPM1B Antibody (1A3-2A4) [H00005495-M01]

ELISA: PP2C beta/PPM1B Antibody (1A3-2A4) [H00005495-M01]

ELISA: PP2C beta/PPM1B Antibody (1A3-2A4) [H00005495-M01] - Detection limit for recombinant GST tagged PPM1B is 0.3 ng/ml as a capture antibody.

Applications for PP2C beta/PPM1B Antibody (1A3-2A4) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactivity against recombinant protein and transfected lysate for WB. It has also been used for IF, RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PP2C beta/PPM1B

The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase has been shown to dephosphorylate cyclin-dependent kinases (CDKs), and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to cause cell-growth arrest or cell death. Alternative splicing results in multiple transcript variants encoding different isoforms. Additional transcript variants have been described, but currently do not represent full-length sequences.

Long Name

Protein Phosphatase 2C beta

Alternate Names

Pp2c2, PP2CB, PPM1B

Entrez Gene IDs

5495 (Human)

Gene Symbol

PPM1B

OMIM

603770 (Human)

UniProt

AAH12002 (Human)

Additional PP2C beta/PPM1B Products

Product Documents for PP2C beta/PPM1B Antibody (1A3-2A4) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for PP2C beta/PPM1B Antibody (1A3-2A4) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PP2C beta/PPM1B Antibody (1A3-2A4) - Azide and BSA Free

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Protocols

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FAQs

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