PP2C beta/PPM1B Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87250

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PP2C beta/PPM1B Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KRNVIEAVYSRLNPHRESDGASDEAEESGSQGKLVEALRQMRINHRGNYRQLLEEMLTSYRLAKVEGEESPAEPAATATSSNSDAGNPVTMQESHTESESGLAEL

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%), Rat (85%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PP2C beta/PPM1B Antibody - BSA Free

Western Blot: PP2C beta/PPM1B Antibody [NBP1-87250]

Western Blot: PP2C beta/PPM1B Antibody [NBP1-87250]

Western Blot: PP2C beta/PPM1B Antibody [NBP1-87250] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunohistochemistry-Paraffin: PP2C beta/PPM1B Antibody [NBP1-87250]

Immunohistochemistry-Paraffin: PP2C beta/PPM1B Antibody [NBP1-87250]

Immunohistochemistry-Paraffin: PP2C beta/PPM1B Antibody [NBP1-87250] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: PP2C beta/PPM1B Antibody [NBP1-87250]

Immunohistochemistry-Paraffin: PP2C beta/PPM1B Antibody [NBP1-87250]

Immunohistochemistry-Paraffin: PP2C beta/PPM1B Antibody [NBP1-87250] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
Immunohistochemistry-Paraffin: PP2C beta/PPM1B Antibody [NBP1-87250]

Immunohistochemistry-Paraffin: PP2C beta/PPM1B Antibody [NBP1-87250]

Immunohistochemistry-Paraffin: PP2C beta/PPM1B Antibody [NBP1-87250] - Staining of human prostate shows strong cytoplasmic positivity in smooth muscle cells.
Immunohistochemistry-Paraffin: PP2C beta/PPM1B Antibody [NBP1-87250]

Immunohistochemistry-Paraffin: PP2C beta/PPM1B Antibody [NBP1-87250]

Immunohistochemistry-Paraffin: PP2C beta/PPM1B Antibody [NBP1-87250] - Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.
PP2C beta/PPM1B Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: PP2C beta/PPM1B Antibody [NBP1-87250]

Immunocytochemistry/ Immunofluorescence: PP2C beta/PPM1B Antibody [NBP1-87250]

Staining of human cell line A-431 shows positivity in nucleoli & cytoplasm.

Applications for PP2C beta/PPM1B Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PP2C beta/PPM1B

PPM1B is encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase has been shown to dephosphorylate cyclin-dependent kinases (CDKs), and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to cause cell-growth arrest or cell death. Alternative splicing results in multiple transcript variants encoding different isoforms. Additional transcript variants have been described, but currently do not represent full-length sequences.

Long Name

Protein Phosphatase 2C beta

Alternate Names

Pp2c2, PP2CB, PPM1B

Gene Symbol

PPM1B

Additional PP2C beta/PPM1B Products

Product Documents for PP2C beta/PPM1B Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PP2C beta/PPM1B Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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